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PMID: 2602366 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Organization of the human lipoprotein lipase gene and evolution of the lipase gene family.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 24 ·1989-12-00 ·Pages 9647-51

Kirchgessner TG, Chuat JC, Heinzmann C, Etienne J, Guilhot S, Svenson K, Ameis D, Pilon C, d'Auriol L, Andalibi A

Abstract

The human lipoprotein lipase gene was cloned and characterized. It is composed of 10 exons spanning approximately equal to 30 kilobases. The first exon encodes the 5'-untranslated region, the signal peptide plus the first two amino acids of the mature protein. The next eight exons encode the remaining 446 amino acids, and the tenth exon encodes the long 3'-untranslated region of 1948 nucleotides. The lipoprotein lipase transcription start site and the sequence of the 5'-flanking region were also determined. We compared the organization of genes for lipoprotein lipase, hepatic lipase, pancreatic lipase, and Drosophila yolk protein 1, which are members of a family of related genes. A model for the evolution of the lipase gene family is presented that involves multiple rounds of gene duplication plus exon-shuffling and intron-loss events.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Exons Gene Library Genes Humans Lipase/genetics Lipoprotein Lipase/genetics Molecular Sequence Data Multigene Family Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Lipase Lipoprotein Lipase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Kirchgessner T G
Department of Medicine, University of California, Los Angeles 90024.
Chuat J C
Heinzmann C
Etienne J
Guilhot S
Svenson K
Ameis D
Pilon C
d'Auriol L
Andalibi A
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.24.9647
Published
1989-12-00
Pages
9647-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298558
Subset
IM
Grants
NHLBI NIH HHS · HL 28481 · United States
Databases
GENBANK
M29549
External Links
PubMed source DOI: 10.1073/pnas.86.24.9647 PMC Full Text View journal details
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