Synthesis and properties of 8-azido-1, N6-etheno adenosine triphosphate--a fluorescent and photosensitive ATP analog.

Nucleic acids research ·Vol. 5 ·No. 4 ·1978-04-00 ·Pages 1345-51

Schäfer HJ, Scheurich P, Rathgeber G, Dose K

Abstract

8-Azido-1,N6-etheno-ATP--a fluorescent and photoreactive ATP analog has been synthesized and characterized by elementary analysis, thin layer chromatography, infrared spectroscopy, proton resonance spectroscopy, UV absorption spectroscopy, and fluorescence spectroscopy. The photolytical decomposition upon irradiation at different pH values is tested.

MeSH Terms

Adenosine Triphosphate/analogs & derivatives Ethenoadenosine Triphosphate/analogs & derivatives,chemical synthesis Fluorescence Hydrogen-Ion Concentration Photolysis Spectrum Analysis

Chemicals

Ethenoadenosine Triphosphate Adenosine Triphosphate

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schäfer H J

Scheurich P

Rathgeber G

Dose K

References (13)

13 references, click to expand

A fluorescent photo-affinity label for cyclic AMP binding proteins.
Biochem Biophys Res Commun. 1976 SEP 20;72(2):575-80 PMID: 10908
8-azido-ADP, a covalent-binding inhibitor of mitochondrial adenine nucleotide translocation.
FEBS Lett. 1976 Apr 15;64(1):185-9 PMID: 131709
Specific photolabelling of beef-heart mitochondrial ATPase by 8-azido-ATP.
Biochim Biophys Acta. 1977 Apr 11;460(1):17-24 PMID: 139919
Photoaffinity labeling of adenosine 3',5'-cyclic monophosphate binding sites of human red cell membranes.
Biochemistry. 1975 Aug 26;14(17):3852-7 PMID: 169887
Interactions of a photo-affinity ATP analog with cation-stimulated adenosine triphosphatases of human red cell membranes.
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3367-71 PMID: 4279407
Chemical synthesis and biological activity of 8-substituted adenosine 3',5'-cyclic monophosphate derivatives.
Biochemistry. 1971 Jun 8;10(12):2390-5 PMID: 4329878
Fluorescent modification of adenosine 3',5'-monophosphate: spectroscopic properties and activity in enzyme systems.
Science. 1972 Jul 21;177(4045):279-80 PMID: 4339302
Fluorescent modification of adenosine-containing coenzymes. Biological activities and spectroscopic properties.
Biochemistry. 1972 Sep 12;11(19):3499-506 PMID: 4340904
Fluorescent adenosine and cytidine derivatives.
Biochem Biophys Res Commun. 1972 Jan 31;46(2):597-604 PMID: 4550694
The 8-azidoadenine analog of S-benzoyl (3'-dephospho) coenzyme A--a photoaffinity label for acyl CoA:glycine N-acyltransferase.
Biochem Biophys Res Commun. 1977 Jun 6;76(3):843-9 PMID: 901448
8-Azidoacenine analogs of NAD+ and FAD. Synthesis and coenzyme properties with NAD+-dependent and FAD-dependent enzymes.
Eur J Biochem. 1976 AUG 1;67(1):223-9 PMID: 9276
Interaction of the photoaffinity label 8-azido-ADP with glutamate dehydrogenase.
FEBS Lett. 1976 Apr 15;64(1):176-80 PMID: 944649
The reaction between thiols and 8-azidoadenosine derivatives.
Nucleic Acids Res. 1976 Sep;3(9):2331-9 PMID: 9623

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.4.1345

Published

1978-04-00

Pages

1345-51

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342082

Subset

External Links

PubMed source DOI: 10.1093/nar/5.4.1345 PMC Full Text View journal details

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Synthesis and properties of 8-azido-1, N6-etheno adenosine triphosphate--a fluorescent and photosensitive ATP analog.

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