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PMID: 2605294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Smoothed orientational order profile of lipid bilayers by 2H-nuclear magnetic resonance.

Biophysical journal ·Vol. 56 ·No. 5 ·1989-11-00 ·Pages 1037-41

Lafleur M, Fine B, Sternin E, Cullis PR, Bloom M

Abstract

A new method has been developed to determine the complete orientational order profile of lipid bilayers using 2H-NMR. The profile is obtained from a single powder spectrum of a lipid which has a saturated chain fully deuteriated. The smoothed order profile is determined directly from the normalized dePaked spectrum assuming a monotonic decrease of the order along the acyl chain. The oscillatory variations of the order at the beginning of the chain are not described by this method. However the smoothed order profile reveals in a straightforward way the crucial features of the anisotropic order of the bilayer.

MeSH Terms
Deuterium Lipid Bilayers Magnetic Resonance Spectroscopy/methods Mathematics Models, Biological Molecular Conformation Phosphatidylcholines
Chemicals
Lipid Bilayers Phosphatidylcholines Deuterium 1-palmitoyl-2-oleoylphosphatidylcholine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lafleur M
Department of Biochemistry, University of British Columbia, Vancouver, Canada.
Fine B
Sternin E
Cullis P R
Bloom M
References (10)
10 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(89)82749-3
Published
1989-11-00
Pages
1037-41
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1280602
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(89)82749-3 PMC Full Text View journal details
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