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PMID: 26108469 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Molecular and neuronal homology between the olfactory systems of zebrafish and mouse.

Scientific reports ·Vol. 5 ·2015-06-25 ·Pages 11487

Saraiva LR, Ahuja G, Ivandic I, Syed AS, Marioni JC, Korsching SI, Logan DW

Abstract

Studies of the two major olfactory organs of rodents, the olfactory mucosa (OM) and the vomeronasal organ (VNO), unraveled the molecular basis of smell in vertebrates. However, some vertebrates lack a VNO. Here we generated and analyzed the olfactory transcriptome of the zebrafish and compared it to the olfactory transcriptomes of mouse to investigate the evolutionary and molecular relationship between single and dual olfactory systems. Our analyses revealed a high degree of molecular conservation, with orthologs of mouse olfactory cell-specific markers and all but one of their chemosensory receptor classes expressed in the single zebrafish olfactory organ. Zebrafish chemosensory receptor genes are expressed across a large dynamic range and their RNA abundance correlates positively with the number of neurons expressing that RNA. Thus we estimate the relative proportions of neuronal sub-types expressing different chemosensory receptors. Receptor repertoire size drives the absolute abundance of different classes of neurons, but we find similar underlying patterns in both species. Finally, we identified novel marker genes that characterize rare neuronal populations in both mouse and zebrafish. In sum, we find that the molecular and cellular mechanisms underpinning olfaction in teleosts and mammals are similar despite 430 million years of evolutionary divergence.

MeSH Terms
Animals Biological Evolution Brain/metabolism Cluster Analysis High-Throughput Nucleotide Sequencing Male Mice Olfactory Mucosa/metabolism Olfactory Receptor Neurons/metabolism Phylogeny RNA/chemistry,metabolism Receptors, Formyl Peptide/classification,metabolism Receptors, Odorant/metabolism Receptors, Progesterone/genetics,metabolism Sequence Analysis, RNA Transcriptome Vomeronasal Organ/metabolism Zebrafish
Chemicals
Receptors, Formyl Peptide Receptors, Odorant Receptors, Progesterone RNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Saraiva Luis R
1] Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton-Cambridge, CB10 1SA, United Kingdom [2] European Bioinformatics Institute (EMBL-EBI), European Molecular Biology Laboratory, Wellcome Trust Genome Campus, Hinxton-Cambridge, CB10 1SD, United Kingdom.
Ahuja Gaurav
Institut für Genetik, Universität zu Köln, Cologne, 50674, Germany.
Ivandic Ivan
Institut für Genetik, Universität zu Köln, Cologne, 50674, Germany.
Syed Adnan S
Institut für Genetik, Universität zu Köln, Cologne, 50674, Germany.
Marioni John C
European Bioinformatics Institute (EMBL-EBI), European Molecular Biology Laboratory, Wellcome Trust Genome Campus, Hinxton-Cambridge, CB10 1SD, United Kingdom.
Korsching Sigrun I
Institut für Genetik, Universität zu Köln, Cologne, 50674, Germany.
Logan Darren W
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton-Cambridge, CB10 1SA, United Kingdom.
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Article Info
Journal
Scientific reports
Abbr.
Sci Rep
ISSN
2045-2322
DOI
10.1038/srep11487
Published
2015-06-25
Epub
2015-00-25
Pages
11487
Language
English
Region
England
NLM ID
101563288
PMCID
PMC4480006
Subset
IM
Grants
Wellcome Trust · 098051 · United Kingdom
External Links
PubMed source DOI: 10.1038/srep11487 PMC Full Text View journal details
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