Home LiteratureArticle Details
PMID: 2613548 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Uptake of exogenous protein tracer in cells of the rat renal papilla.

Histochemistry ·Vol. 93 ·No. 1 ·1989-00-00 ·Pages 63-8

Sundelin B, Bohman SO

Abstract

In order to study the phagocytic potential of different cell types of the rat renal papilla with special emphasis on interstitial cells, horseradish peroxidase (HRP) (8 mg/100 g body weight) was injected intravenously into adult rats. The distribution of peroxidase was studied in animals perfusion-fixed 60 and 180 min after injection and was found to be similar after both time intervals. The epithelial cells of the collecting ducts took up the largest amounts of the tracer. HRP was mainly located in large lysosome-like bodies in the basal part of the cytoplasm, suggesting peritubular uptake from the interstitial space. However, small amounts of the tracer were also seen in apical vesicles close to the luminal plasma membrane. The interstitial cells of peroxidase-injected animals were ultrastructurally altered and had large irregular invaginations of the cell membrane. The cells had taken up only small amounts of the tracer which were located in small round lysosome-like bodies. Thus, the interstitial cells displays no macrophage characteristics, either in the native state or when challenged with an extracellular protein.

MeSH Terms
Animals Horseradish Peroxidase/pharmacokinetics,pharmacology Kidney Medulla/drug effects,metabolism,ultrastructure Male Microscopy, Electron/methods Peroxidases/pharmacokinetics Rats Rats, Inbred Strains
Chemicals
Horseradish Peroxidase Peroxidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sundelin B
Department of Pathology, Huddinge Hospital, Sweden.
Bohman S O
References (21)
21 references, click to expand
  1. The pinocytic rate of activated macrophages.
    J Exp Med. 1975 Nov 1;142(5):1150-64 PMID: 53258
  2. Cytochemical observations on the transport of horseradish peroxidase in different segments of the nephron.
    Histochem J. 1972 Nov;4(6):517-29 PMID: 4120448
  3. Subcellular localization of the prostaglandin system in the rabbit renal papilla.
    Acta Physiol Scand. 1972 Feb;84(2):231-46 PMID: 5015190
  4. Fine structure of the rat renal papilla.
    Am J Anat. 1966 May;118(3):685-721 PMID: 5956103
  5. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
    J Histochem Cytochem. 1966 Apr;14(4):291-302 PMID: 5962951
  6. Binding and receptor-mediated endocytosis of pregnancy zone protein-proteinase complex in rat macrophages.
    Biochim Biophys Acta. 1987 Oct 1;930(3):297-303 PMID: 2443184
  7. Identification of a novel cell type in peripheral lymphoid organs of mice. II. Functional properties in vitro.
    J Exp Med. 1974 Feb 1;139(2):380-97 PMID: 4589990
  8. Identification of a novel cell type in peripheral lymphoid organs of mice. V. Purification of spleen dendritic cells, new surface markers, and maintenance in vitro.
    J Exp Med. 1979 Jan 1;149(1):1-16 PMID: 762493
  9. Mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Identification of resident macrophages in renal medullary and cortical interstitium and the juxtaglomerular complex.
    J Exp Med. 1983 May 1;157(5):1704-9 PMID: 6854206
  10. Albumin multiplier in kidney vasa recta analyzed by microspectrophotometry of T-1824.
    Am J Physiol. 1967 Nov;213(5):1233-43 PMID: 6054870
  11. Ultrastructure and biochemical properties of subcellular fractions from rat renal medulla.
    J Ultrastruct Res. 1972 Feb;38(3):225-45 PMID: 4333864
  12. Vascular leakage induced by horseradish peroxidase in the rat.
    Proc Soc Exp Biol Med. 1967 Nov;126(2):557-61 PMID: 4383959
  13. The ultrastructure of the rat renal medulla as observed after improved fixation methods.
    J Ultrastruct Res. 1974 Jun;47(3):329-60 PMID: 4209523
  14. Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution.
    J Exp Med. 1973 May 1;137(5):1142-62 PMID: 4573839
  15. Peroxidase uptake by renal papillary cells of normal and hypokalemic rats.
    Lab Invest. 1973 Mar;28(3):305-12 PMID: 4693925
  16. Interstitial cells of the renal medulla.
    J Ultrastruct Res. 1966 Aug;15(5):589-613 PMID: 5911654
  17. On lipid droplets in renal interstitial cells. IV. Isolation and identification.
    Z Zellforsch Mikrosk Anat. 1969 May 23;97(2):274-84 PMID: 4907379
  18. Observations on the fine structure and peroxidase cytochemistry of normal rat liver Kupffer cells.
    J Ultrastruct Res. 1974 Mar;46(3):393-426 PMID: 4363811
  19. The renomedullary system of blood pressure control.
    Am J Med Sci. 1988 Apr;295(4):231-3 PMID: 3284345
  20. Demonstration and characterization of Ia-positive dendritic cells in the interstitial connective tissues of rat heart and other tissues, but not brain.
    J Exp Med. 1981 Aug 1;154(2):347-61 PMID: 6943285
  21. Comparative analysis of the concentration of injected horseradish peroxidase in cytoplasmic granules of the kidney cortex, in the blood, urine, and liver.
    J Cell Biol. 1971 Mar;48(3):620-32 PMID: 4928550
Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00266848
Published
1989-00-00
Pages
63-8
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00266848
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2