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PMID: 2615791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A versiconal hemiacetal acetate converting enzyme in aflatoxin biosynthesis.

Mycopathologia ·Vol. 107 ·No. 2-3 ·1989-09-00 ·Pages 121-6

Hsieh DP, Wan CC, Billington JA

Abstract

Conversion of the aflatoxin biosynthetic intermediate versiconal hemiacetal acetate (VHA) in a cell free extract of Aspergillus parasiticus ATCC 15517 is investigated. The enzymatic reaction is monitored by a method using high performance liquid chromatography (HPLC). The major product of the enzymatic reaction is a water soluble compound not chloroform-extractable at pH 7.5. The product becomes chloroform extractable upon acidification of the reaction medium and is separated and quantitated by reversed-phase HPLC. It is tentatively identified as 'versiconal hemiacetal alcohol,' which is converted to versicolorin C (VC) upon acid treatment.

MeSH Terms
Aflatoxins/biosynthesis Anthraquinones/metabolism Aspergillus/enzymology Carboxylic Ester Hydrolases/metabolism Chromatography, High Pressure Liquid Hydrogen-Ion Concentration
Chemicals
Aflatoxins Anthraquinones versiconal hemiacetal acetate versicolorin A Carboxylic Ester Hydrolases versiconal hemiacetal acetate converting enzyme versicolorin C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hsieh D P
Department of Environmental Toxicology, University of California, Davis 95616.
Wan C C
Billington J A
References (9)
9 references, click to expand
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Article Info
Journal
Mycopathologia
Abbr.
Mycopathologia
ISSN
0301-486X
DOI
10.1007/BF00707548
Published
1989-09-00
Pages
121-6
Language
English
Region
Netherlands
NLM ID
7505689
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00707548 View journal details
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