Pseudo-nitzschia multiseries belongs to Baciuariophyta, which can produce the neurotoxin known as domoic acid, a toxin that can cause amnesic shellfish poisoning in human beings. The research aspects mainly focus on its toxicity and diversity. However, there is no mitochondrial genome information about P. multiseries. The complete mitochondrial genome of P. multiseries was obtained through amplifying. The mitogenome is 46 283 bp in length with 31.04% GC content. It has a typical cyclic structure. The genome contains 37 protein-coding genes, including unidentified open reading frames, 23 tRNAs and 2rRNAs. Four group II introns were found in rps5, cob, cox1 and cox2. The co-linear analysis result exhibited existence of gene modules re-arrangements and inversions among P. multiseries, Synedra acus and Thalassiosira pseudonana. The gene contents comparison showed they have high similarities. Phylogeny results suggest P. multiseries has a close evolutionary relationship with T. pseudonana.
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