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PMID: 2643116 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Dynamic aspects of intermediate filament networks in BHK-21 cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 2 ·1989-01-00 ·Pages 549-53

Vikstrom KL, Borisy GG, Goldman RD

Abstract

A procedure was developed for the conjugation of vimentin with biotin. Biotinylated vimentin was then microinjected into BHK-21 cells and the fate of the labeled protein was determined at various times postinjection by indirect immunofluorescence. Microinjected vimentin could be traced through a specific sequence of morphological changes ultimately resulting in the formation of a filamentous network. The injected protein was first detected in spots dispersed throughout the cytoplasm. Subsequently, these spots appeared to cluster near the nucleus where they merged into a diffuse "cap." This cap coincided with a concentration of endogenous intermediate filaments and eventually gave rise to a filamentous network that was coincident with the endogenous intermediate filament network as determined by double-label immunofluorescence. The results indicate that the incorporation of exogenous vimentin into a filamentous network is initiated in a perinuclear region and progresses in a polarized fashion toward the cell surface.

MeSH Terms
Animals Biotin Cell Line Cytoskeleton/metabolism Fluorescent Antibody Technique Intermediate Filaments/metabolism Microinjections Vimentin/metabolism
Chemicals
Vimentin Biotin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vikstrom K L
Department of Cell Biology and Anatomy, Northwestern University Medical School, Chicago, IL 60611.
Borisy G G
Goldman R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.2.549
Published
1989-01-00
Pages
549-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286509
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.2.549 PMC Full Text View journal details
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