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PMID: 2646285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Specific amino acid residues in both the PstB and PstC proteins are required for phosphate transport by the Escherichia coli Pst system.

Journal of bacteriology ·Vol. 171 ·No. 3 ·1989-03-00 ·Pages 1531-4

Cox GB, Webb D, Rosenberg H

Abstract

Three mutant alleles of the pstC gene and one mutant allele of the pstB gene were produced by site-directed mutagenesis. The pstC gene encodes an integral membrane protein of the phosphate-specific transport (Pst) system of Escherichia coli. The amino acid substitutions resulting from the pstC gene mutations, Arg-237----Gln, Glu-240----Gln, or a combination of both, caused the loss of phosphate transport through the Pst system, but the alkaline phosphatase activity remained repressed. The pstB gene encodes a peripheral membrane protein of the Pst system which carries a putative nucleotide-binding site. The amino acid substitutions Gly-48----Ile and Lys-49----Gln, resulting from the pstB mutations, caused the loss of phosphate transport through the Pst system and the derepression of alkaline phosphatase activity. The residues Gly-48 and Lys-49 are key residues in the putative nucleotide-binding site.

MeSH Terms
Alkaline Phosphatase/metabolism Alleles Base Sequence Carrier Proteins/genetics,metabolism Escherichia coli/genetics Genes Genes, Bacterial Genotype Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutation Oligonucleotide Probes Phosphate-Binding Proteins Phosphates/metabolism
Chemicals
Carrier Proteins Membrane Proteins Oligonucleotide Probes Phosphate-Binding Proteins Phosphates Alkaline Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cox G B
Division of Biochemical Sciences, John Curtin School of Medical Research, Australian National University, Canberra City A.C.T.
Webb D
Rosenberg H
References (17)
17 references, click to expand
  1. A ne type of alkaline phosphatase-negative mutants in Escherichia coli K12.
    Mol Gen Genet. 1973 Mar 27;122(1):53-60 PMID: 4573262
  2. Arg-220 of the PstA protein is required for phosphate transport through the phosphate-specific transport system in Escherichia coli but not for alkaline phosphatase repression.
    J Bacteriol. 1988 May;170(5):2283-6 PMID: 2896188
  3. Metabolite transport in mutants of Escherichia coli K12 defective in electron transport and coupled phosphorylation.
    Biochem J. 1975 Feb;146(2):417-23 PMID: 125586
  4. Uniform nomenclature for bacterial plasmids: a proposal.
    Bacteriol Rev. 1976 Mar;40(1):168-89 PMID: 1267736
  5. Mitomycin C-induced expression of trpA of Salmonella typhimurium inserted into the plasmid ColE1.
    J Bacteriol. 1977 Jan;129(1):388-94 PMID: 318646
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. Transport of iron into bacterial cells.
    Methods Enzymol. 1979;56:388-94 PMID: 379514
  8. Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli.
    Genetics. 1980 Oct;96(2):353-66 PMID: 7021308
  9. Genetic analysis of mutants affected in the Pst inorganic phosphate transport system.
    J Bacteriol. 1981 Oct;148(1):1-9 PMID: 7026529
  10. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  11. Mutations in the uncE gene affecting assembly of the c-subunit of the adenosine triphosphatase of Escherichia coli.
    Biochem J. 1983 Jun 1;211(3):717-26 PMID: 6309138
  12. Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships.
    J Bacteriol. 1985 Jan;161(1):189-98 PMID: 3881386
  13. Nucleotide binding by membrane components of bacterial periplasmic binding protein-dependent transport systems.
    EMBO J. 1985 Apr;4(4):1033-9 PMID: 3926486
  14. Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli.
    J Mol Biol. 1985 Jul 20;184(2):241-50 PMID: 2993631
  15. Directed mutagenesis of the beta-subunit of F1-ATPase from Escherichia coli.
    J Biol Chem. 1987 Jun 15;262(17):8022-6 PMID: 2885316
  16. The proton pore in the Escherichia coli F0F1-ATPase: a requirement for arginine at position 210 of the a-subunit.
    Biochim Biophys Acta. 1987 Dec 17;894(3):399-406 PMID: 2891376
  17. The relationship between the phosphate-binding protein and a regulator gene product from Escherichia coli.
    Biochim Biophys Acta. 1974 May 10;351(1):77-86 PMID: 4599976
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.3.1531-1534.1989
Published
1989-03-00
Pages
1531-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209777
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.3.1531-1534.1989 PMC Full Text View journal details
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