A novel prostaglandin is the major product of arachidonic acid metabolism in rabbit heart.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 1 ·1977-01-00 ·Pages 101-5

Isakson PC, Raz A, Denny SE, Pure E, Needleman P

Abstract

The prostaglandins (PGs) released from the heart have generally been characterized as resembling PGE by bioassay techniques. The major PG formed from [14C]arachidonate (C20:4) by the isolated perfused rabbit heart has chromatographic mobility similar to that of PGE2 in most solvent systems. However, additional analysis of this radioactive "PGE" peak suggests that two substances are formed by the heart and migrate like PGE2: one has chemical properties similar to those of authentic PGE2 and the other is a novel PG. The unknown compound is the major PG formed by the heart from either exogenous arachidonate or hormonal stimulation of PG biosynthesis. The novel PG produced by the heart may be identical with either 6(9)-oxy-PGF or 6-keto-PGF1 alpha.

MeSH Terms

8,11,14-Eicosatrienoic Acid/metabolism Animals Arachidonic Acids/metabolism Gastric Mucosa/metabolism Male Myocardium/metabolism Prostaglandins/analysis,biosynthesis Rabbits Rats

Chemicals

Arachidonic Acids Prostaglandins 8,11,14-Eicosatrienoic Acid

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Isakson P C

Raz A

Denny S E

Pure E

Needleman P

References (17)

17 references, click to expand

Hormone interactions in the isolated rabbit heart. Synthesis and coronary vasomotor effects of prostaglandins, angiotensin, and bradykinin.
Circ Res. 1975 Dec;37(6):802-8 PMID: 1192572
Letter: Isolation, structure, and biosynthesis of 6-ketoprostaglandin F1 alpha in the rat stomach.
J Am Chem Soc. 1976 Apr 14;98(8):2348-9 PMID: 1254872
Phospholipid precursors of prostaglandins.
Biochim Biophys Acta. 1968 Oct 22;164(2):426-9 PMID: 5721037
PROSTAGLANDINS AND RELATED FACTORS: XIX. THIN-LAYER CHROMATOGRAPHY OF PROSTAGLANDINS.
J Lipid Res. 1964 Jan;5:117-20 PMID: 14173316
An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation.
Nature. 1976 Oct 21;263(5579):663-5 PMID: 802670
The synthesis and function of prostaglandins in the heart.
Fed Proc. 1976 Oct;35(12):2376-81 PMID: 786741
Selective incorporation of 14C-arachidonic acid into the phospholipids of intact tissues and subsequent metabolism to 14C-prostaglandins.
Prostaglandins. 1976 Nov;12(5):739-48 PMID: 981699
A novel prostaglandin derivative formed from arachidonic acid by rat stomach homogenates.
Biochemistry. 1971 Sep 28;10(20):3657-64 PMID: 5096392
Preparative thin-layer and column chromatography of prostaglandins.
J Lipid Res. 1969 May;10(3):316-9 PMID: 4306615
Quantitative conversion of PGA or PGE to PGB.
Prostaglandins. 1972 Feb;1(2):167-8 PMID: 4660070
Prostaglandins and the regulation of uterine blood flow in pregnancy.
Nature. 1974 May 3;249(452):57-8 PMID: 4364355
Modification of basal release of prostaglandins from rabbit isolated hearts.
Prostaglandins. 1974 Sep 25;7(6):473-86 PMID: 4414700
Hypoxia causes prostaglandin release from perfused rabbit hearts.
Acta Physiol Scand. 1974 May;91(1):133-5 PMID: 4835718
Isolation and properties of intermediates in prostaglandin biosynthesis.
Biochim Biophys Acta. 1973 Dec 20;326(3):448-61 PMID: 4776443
The importance of phospholipase-A2 in prostaglandin biosynthesis.
Biochem Pharmacol. 1976 Feb 1;25(3):285-91 PMID: 817726
Mechanism and modification of bradykinin-induced coronary vasodilation.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2060-3 PMID: 1056012
Relationship between oxygen tension, coronary vasodilation and prostaglandin biosynthesis in the isolated rabbit heart.
Prostaglandins. 1975 Jan;9(1):123-34 PMID: 1135423

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.1.101

Published

1977-01-00

Pages

101-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC393205

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.1.101 PMC Full Text View journal details

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A novel prostaglandin is the major product of arachidonic acid metabolism in rabbit heart.

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