In vitro proteolytic cleavage of the Escherichia coli Ada protein by the ompT gene product.

Journal of bacteriology ·Vol. 171 ·No. 4 ·1989-04-00 ·Pages 2249-51

Sedgwick B

Abstract

Down regulation of the adaptive response to alkylation damage in Escherichia coli has been proposed to occur by proteolytic cleavage of the regulatory Ada protein. In this paper, it is shown that proteolysis of the Ada protein as observed in cell extracts is caused by the ompT gene product. This protease, however, was not involved in switching off the adaptive response in vivo.

MeSH Terms

Bacterial Outer Membrane Proteins/physiology Escherichia coli/physiology Gene Expression Regulation Genes, Bacterial Hydrolysis Methylation Peptide Hydrolases/physiology Transcription Factors/physiology

Chemicals

Bacterial Outer Membrane Proteins Transcription Factors Peptide Hydrolases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Sedgwick B

Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom.

References (20)

20 references, click to expand

A new pathway for DNA repair in Escherichia coli.
Nature. 1977 May 19;267(5608):281-3 PMID: 325420
Alteration of the carboxyl-terminal domain of Ada protein influences its inducibility, specificity, and strength as a transcriptional activator.
J Bacteriol. 1988 Nov;170(11):5263-71 PMID: 3141384
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
Repair of alkylated DNA in Escherichia coli. Physical properties of O6-methylguanine-DNA methyltransferase.
J Biol Chem. 1982 Nov 25;257(22):13776-80 PMID: 6754717
Microbial envelope proteins related to iron.
Annu Rev Microbiol. 1982;36:285-309 PMID: 6293371
omp T: Escherichia coli K-12 structural gene for protein a (3b).
J Bacteriol. 1983 Feb;153(2):1104-6 PMID: 6337119
Sequence of the regulatory region of omp T, the gene specifying major outer membrane protein a (3b) of Escherichia coli K-12: implications for regulation and processing.
Mol Gen Genet. 1984;193(3):414-21 PMID: 6323918
Induction of resistance to alkylating agents in E. coli: the ada+ gene product serves both as a regulatory protein and as an enzyme for repair of mutagenic damage.
EMBO J. 1984 Sep;3(9):2151-7 PMID: 6092060
Active site and complete sequence of the suicidal methyltransferase that counters alkylation mutagenesis.
Proc Natl Acad Sci U S A. 1985 May;82(9):2688-92 PMID: 3887409
Methyl phosphotriesters in alkylated DNA are repaired by the Ada regulatory protein of E. coli.
Nucleic Acids Res. 1985 Apr 25;13(8):2683-98 PMID: 2987862
Sequences of the E. coli uvrB gene and protein.
Nucleic Acids Res. 1986 Mar 25;14(6):2637-50 PMID: 3515321
The intracellular signal for induction of resistance to alkylating agents in E. coli.
Cell. 1986 Apr 25;45(2):315-24 PMID: 3009022
Regulatory mechanisms for induction of synthesis of repair enzymes in response to alkylating agents: ada protein acts as a transcriptional regulator.
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6297-301 PMID: 3529081
Complementation of sensitivity to alkylating agents in Escherichia coli and Chinese hamster ovary cells by expression of a cloned bacterial DNA repair gene.
EMBO J. 1986 Dec 1;5(12):3195-200 PMID: 3545815
Proteolytic processing of the Ada protein that repairs DNA O6-methylguanine residues in E. coli.
Mutat Res. 1987 Mar;183(2):123-7 PMID: 3547105
ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification.
J Bacteriol. 1988 Mar;170(3):1245-53 PMID: 3277950
Functional domains and methyl acceptor sites of the Escherichia coli ada protein.
J Biol Chem. 1988 Mar 25;263(9):4430-3 PMID: 3162236
Repair of DNA-containing pyrimidine dimers.
FASEB J. 1988 Aug;2(11):2696-701 PMID: 3294078
Regulation and expression of the adaptive response to alkylating agents.
Annu Rev Biochem. 1988;57:133-57 PMID: 3052269
Modification of a ferric enterobactin receptor protein from the outer membrane of Escherichia coli.
Biochem Biophys Res Commun. 1978 Jul 28;83(2):739-46 PMID: 151543

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.171.4.2249-2251.1989

Published

1989-04-00

Pages

2249-51

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC209889

Subset

External Links

PubMed source DOI: 10.1128/jb.171.4.2249-2251.1989 PMC Full Text View journal details

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In vitro proteolytic cleavage of the Escherichia coli Ada protein by the ompT gene product.

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