Comparison of innate immunity mediators in peritoneal fluid and spleen between young and aged rats.

Aging of the innate immune system can result in a wide array of decreasing biological function.,We investigated differences between young and old mice in the expression of the pattern-recognition receptors (PRRs).,mRNA levels for PRRs were quantified and compared in peritoneal fluid and spleens from old (36 months old) and young (1 month old) Wistar white rats (n = 8/group) using real-time reverse transcription-polymerase chain reaction.,In old rats, TLR-5 and -7 were decreased in peritoneal fluid, whereas TLR 4/6/9 and Syk were increased in the spleen (p < 0.05). In young rats, TLR 2/4, dectin-1, and Trem-1 were increased in peritoneal fluid and decreased in the spleen (p < 0.05), but TLR 1/3/7/9/10 and Syk were vice versa (p < 0.05).,Several parameters related to innate immunity may change with aging.,Different expressions of mRNA for several PRRs are suggesting changes in innate immune responses in association with aging.