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PMID: 2651421 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Analysis and comparison of nucleotide sequences encoding the genes for [NiFe] and [NiFeSe] hydrogenases from Desulfovibrio gigas and Desulfovibrio baculatus.

Journal of bacteriology ·Vol. 171 ·No. 5 ·1989-05-00 ·Pages 2894-9

Voordouw G, Menon NK, LeGall J, Choi ES, Peck HD, Przybyla AE

Abstract

The nucleotide sequences encoding the [NiFe] hydrogenase from Desulfovibrio gigas and the [NiFeSe] hydrogenase from Desulfovibrio baculatus (N.K. Menon, H.D. Peck, Jr., J. LeGall, and A.E. Przybyla, J. Bacteriol. 169:5401-5407, 1987; C. Li, H.D. Peck, Jr., J. LeGall, and A.E. Przybyla, DNA 6:539-551, 1987) were analyzed by the codon usage method of Staden and McLachlan. The reported reading frames were found to contain regions of low codon probability which are matched by more probable sequences in other frames. Renewed nucleotide sequencing showed the probable frames to be correct. The corrected sequences of the two small and large subunits share a significant degree of sequence homology. The small subunit, which contains 10 conserved cysteine residues, is likely to coordinate at least 2 iron-sulfur clusters, while the finding of a selenocysteine codon (TGA) near the 3' end of the [NiFeSe] large-subunit gene matched by a regular cysteine codon (TGC) in the [NiFe] large-subunit gene indicates the presence of some of the ligands to the active-site nickel in the large subunit.

MeSH Terms
Amino Acid Sequence Codon DNA, Bacterial/genetics Desulfovibrio/genetics Genes, Bacterial Hydrogenase/genetics Iron Metalloproteins/genetics Molecular Sequence Data Nickel Selenium Sequence Homology, Nucleic Acid
Chemicals
Codon DNA, Bacterial Metalloproteins Nickel Iron Hydrogenase Selenium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Voordouw G
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Menon N K
LeGall J
Choi E S
Peck H D
Przybyla A E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.5.2894-2899.1989
Published
1989-05-00
Pages
2894-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209983
Subset
IM
Grants
NIGMS NIH HHS · GM34903 · United States
External Links
PubMed source DOI: 10.1128/jb.171.5.2894-2899.1989 PMC Full Text View journal details
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