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PMID: 2654938 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence, tissue distribution, and differential expression of mRNA for a putative insulin-responsive glucose transporter in mouse 3T3-L1 adipocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 9 ·1989-05-00 ·Pages 3150-4

Kaestner KH, Christy RJ, McLenithan JC, Braiterman LT, Cornelius P, Pekala PH, Lane MD

Abstract

The cDNAs for two putative glucose transporters from mouse 3T3-L1 adipocytes were isolated and sequenced. One of these cDNAs encodes the murine homolog of the human hepG2/erythrocyte glucose transporter, termed GT1. GT1 mRNA is most abundant in mouse brain and is expressed in both 3T3-L1 preadipocytes and adipocytes. The other cDNA encodes a glucose transporter-like protein, termed GT2, that has a unique amino acid sequence and tissue distribution. GT2 cDNA encodes a protein with 63% amino acid sequence identity and a similar structural organization to GT1. GT2 mRNA is found at high levels in mouse skeletal muscle, heart, and adipose tissue, all of which exhibit insulin-stimulated glucose uptake. GT2 mRNA is absent from 3T3-L1 preadipocytes but is induced dramatically during differentiation into adipocytes. This increase in mRNA content correlates closely with the acquisition of insulin-stimulated glucose uptake. We propose that GT2 is an insulin-regulated glucose transporter.

MeSH Terms

Adipose Tissue/drug effects,metabolism Amino Acid Sequence Animals Base Sequence Cell Differentiation Cell Line DNA/genetics Gene Expression Regulation Glucose/metabolism Humans Insulin/pharmacology Mice Molecular Sequence Data Monosaccharide Transport Proteins/genetics RNA, Messenger/analysis,genetics Sequence Homology, Nucleic Acid Tissue Distribution

Chemicals

Insulin Monosaccharide Transport Proteins RNA, Messenger DNA Glucose

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Kaestner K H

Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Christy R J

McLenithan J C

Braiterman L T

Cornelius P

Pekala P H

Lane M D

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.9.3150

Published

1989-05-00

Pages

3150-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC287083

Subset

Grants

NIDDK NIH HHS · DK-14574 · United States

NIGMS NIH HHS · GM-00184 · United States

NIGMS NIH HHS · GM32892 · United States

Databases

GENBANK

J04557, M23383

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1073/pnas.86.9.3150 PMC Full Text View journal details

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Sequence, tissue distribution, and differential expression of mRNA for a putative insulin-responsive glucose transporter in mouse 3T3-L1 adipocytes.

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