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PMID: 26595419 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Conserved mRNA-binding proteomes in eukaryotic organisms.

Nature structural & molecular biology ·Vol. 22 ·No. 12 ·2015-12-00 ·Pages 1027-33

Matia-González AM, Laing EE, Gerber AP

Abstract

RNA-binding proteins (RBPs) are essential for post-transcriptional regulation of gene expression. Recent high-throughput screens have dramatically increased the number of experimentally identified RBPs; however, comprehensive identification of RBPs within living organisms is elusive. Here we describe the repertoire of 765 and 594 proteins that reproducibly interact with polyadenylated mRNAs in Saccharomyces cerevisiae and Caenorhabditis elegans, respectively. Furthermore, we report the differential association of mRNA-binding proteins (mRPBs) upon induction of apoptosis in C. elegans L4-stage larvae. Strikingly, most proteins composing mRBPomes, including components of early metabolic pathways and the proteasome, are evolutionarily conserved between yeast and C. elegans. We speculate, on the basis of our evidence that glycolytic enzymes bind distinct glycolytic mRNAs, that enzyme-mRNA interactions relate to an ancient mechanism for post-transcriptional coordination of metabolic pathways that perhaps was established during the transition from the early 'RNA world' to the 'protein world'.

MeSH Terms
Animals Caenorhabditis elegans/chemistry,metabolism Conserved Sequence Gene Expression Regulation Proteome/analysis RNA, Messenger/metabolism RNA-Binding Proteins/analysis,genetics Saccharomyces cerevisiae/chemistry,metabolism
Chemicals
Proteome RNA, Messenger RNA-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Matia-González Ana M
Department of Microbial and Cellular Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.
Laing Emma E
Department of Microbial and Cellular Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.
Gerber André P
Department of Microbial and Cellular Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.3128
Published
2015-12-00
Epub
2015-00-23
Pages
1027-33
Language
English
Region
United States
NLM ID
101186374
PMCID
PMC5759928
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/K009303/1 · United Kingdom
External Links
PubMed source DOI: 10.1038/nsmb.3128 PMC Full Text View journal details
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