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PMID: 2661545 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Use of the isocitrate dehydrogenase structural gene for attachment of e14 in Escherichia coli K-12.

Journal of bacteriology ·Vol. 171 ·No. 7 ·1989-07-00 ·Pages 4083-4

Hill CW, Gray JA, Brody H

Abstract

The e14 element appears to be integrated into the Escherichia coli K-12 isocitrate dehydrogenase structural gene (icd). In being integrated, it replaced the last 52 codons of the gene with a closely related sequence. The two versions of the icd gene produce proteins of the same length but differ by 12 base substitutions that would cause two conservative amino acid replacements.

MeSH Terms
Amino Acid Sequence Attachment Sites, Microbiological Bacterial Proteins/genetics,isolation & purification Base Sequence Escherichia coli/genetics Genes Genes, Bacterial Isocitrate Dehydrogenase/genetics,isolation & purification Lysogeny Molecular Sequence Data
Chemicals
Bacterial Proteins Isocitrate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hill C W
Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania 17033.
Gray J A
Brody H
References (10)
10 references, click to expand
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    Science. 1983 Jul 22;221(4608):378-80 PMID: 6346486
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    J Bacteriol. 1984 May;158(2):517-22 PMID: 6233259
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  7. Inactivation of isocitrate dehydrogenase by phosphorylation is mediated by the negative charge of the phosphate.
    J Biol Chem. 1987 Aug 5;262(22):10422-5 PMID: 3112144
  8. Integration of satellite bacteriophage P4 in Escherichia coli. DNA sequences of the phage and host regions involved in site-specific recombination.
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  9. Attachment site of the genetic element e14.
    J Bacteriol. 1988 May;170(5):2040-4 PMID: 3283103
  10. Excision and reintegration of the Escherichia coli K-12 chromosomal element e14.
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.7.4083-4084.1989
Published
1989-07-00
Pages
4083-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210168
Subset
IM
Grants
PHS HHS · 16329 · United States
NCRR NIH HHS · P4 1 RR01685 · United States
Databases
GENBANK
J02799, M19683, M19693
External Links
PubMed source DOI: 10.1128/jb.171.7.4083-4084.1989 PMC Full Text View journal details
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