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PMID: 2661563 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion.

The Journal of cell biology ·Vol. 108 ·No. 6 ·1989-06-00 ·Pages 2435-47

Behrens J, Mareel MM, Van Roy FM, Birchmeier W

Abstract

The generation of invasiveness in transformed cells represents an essential step of tumor progression. We show here, first, that nontransformed Madin-Darby canine kidney (MDCK) epithelial cells acquire invasive properties when intercellular adhesion is specifically inhibited by the addition of antibodies against the cell adhesion molecule uvomorulin; the separated cells then invade collagen gels and embryonal heart tissue. Second, MDCK cells transformed with Harvey and Moloney sarcoma viruses are constitutively invasive, and they were found not to express uvomorulin at their cell surface. These data suggest that the loss of adhesive function of uvomorulin (which is identical to E-cadherin and homologous to L-CAM) is a critical step in the promotion of epithelial cells to a more malignant, i.e., invasive, phenotype. Similar modulation of intercellular adhesion might also occur during invasion of carcinoma cells in vivo.

MeSH Terms
Antibodies, Monoclonal Antigen-Antibody Reactions Antigens, Surface/physiology Cadherins Cell Adhesion Cell Adhesion Molecules Cell Line Cell Transformation, Viral Cells, Cultured Collagen Epithelium/pathology Fluorescent Antibody Technique In Vitro Techniques Membrane Glycoproteins/physiology Microscopy, Electron Myocardium/cytology Neoplasm Metastasis Neoplasms, Experimental/pathology
Chemicals
Antibodies, Monoclonal Antigens, Surface Cadherins Cell Adhesion Molecules Membrane Glycoproteins Collagen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Behrens J
Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Tübingen, Federal Republic of Germany.
Mareel M M
Van Roy F M
Birchmeier W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.6.2435
Published
1989-06-00
Pages
2435-47
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115620
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.108.6.2435 PMC Full Text View journal details
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