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PMID: 2670564 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A DNA binding protein that recognizes oligo(dA).oligo(dT) tracts.

The EMBO journal ·Vol. 8 ·No. 6 ·1989-06-00 ·Pages 1867-77

Winter E, Varshavsky A

Abstract

Oligo(dA).oligo(dT) tracts are common intergenic sequences in many organisms. In the yeast Saccharomyces cerevisiae, these sequences have been shown to influence transcription of adjacent genes. We have purified an oligo(dA).oligo(dT)-binding protein from S. cerevisiae and cloned its gene. This protein, which has been named datin, requires at least 9-11 bp of oligo(dA).oligo(dT) DNA for high affinity binding. The gene for datin (the DAT gene) encodes a 248-residue protein which contains a number of repeated sequence motifs. Datin purified from yeast corresponds to the N-terminal half of the DAT gene product. Null mutants in the DAT gene are viable but phenotypically distinguishable from congenic wild-type strains. We discuss unusual structural features and biochemical properties of datin in relation to its possible functions.

MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites Cloning, Molecular DNA, Fungal/genetics DNA-Binding Proteins/genetics,metabolism Dopamine Plasma Membrane Transport Proteins Escherichia coli/genetics Gene Expression Regulation Genes, Fungal Membrane Glycoproteins Membrane Transport Proteins Molecular Sequence Data Mutation Nerve Tissue Proteins Oligodeoxyribonucleotides/metabolism Poly dA-dT/metabolism Polydeoxyribonucleotides/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Transcription, Genetic
Chemicals
DAT1 protein, S cerevisiae DNA, Fungal DNA-Binding Proteins Dopamine Plasma Membrane Transport Proteins Membrane Glycoproteins Membrane Transport Proteins Nerve Tissue Proteins Oligodeoxyribonucleotides Polydeoxyribonucleotides Saccharomyces cerevisiae Proteins oligo(dA-dT) Poly dA-dT
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Winter E
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Varshavsky A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-06-00
Pages
1867-77
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401036
Subset
IM
Grants
NCI NIH HHS · CA43309 · United States
NIGMS NIH HHS · GM10830 · United States
NIGMS NIH HHS · GM33401 · United States
Databases
GENBANK
X15478
External Links
PubMed source PMC Full Text View journal details
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