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PMID: 2670888 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The yopM gene of Yersinia pestis encodes a released protein having homology with the human platelet surface protein GPIb alpha.

Journal of bacteriology ·Vol. 171 ·No. 9 ·1989-09-00 ·Pages 4623-32

Leung KY, Straley SC

Abstract

In Yersinia pestis KIM, there are 11 Yops (yersinial outer membrane proteins) encoded by the low-Ca2+ response virulence plasmid pCD1. Only Yops M and N are found in easily detectable amounts in the culture medium. In this study, we located and characterized the yopM gene to obtain clues about its role in the virulence of Y. pestis. Rabbit antibody was raised against Yops M and H, copurified from the supernatant of Y. pseudotuberculosis 43(pGW600, pCD1 yopE::Mu dI1[Apr lac]). This antiserum was adsorbed with an Escherichia coli clone that strongly expressed YopH. The resulting YopM-specific antibody was used to screen a HindIII library of pCD1. HindIII-F and several subclones from it expressed YopM in E. coli minicells. A DNA fragment of 1.39 kilobases from HindIII-F was sequenced and found to contain a 367-amino-acid open reading frame capable of encoding a protein with molecular mass (41,566 daltons) and isoelectric point (4.06) similar to those of YopM. The +1 site of the yopM gene was determined by primer extension. The DNA sequence contained repeating structures: 11 pairs of exact direct repeats, two exact inverted repeats, and three palindromes, ranging from 10 to 42 bases in size. One consensus 14-amino-acid sequence was repeated six times in the predicted protein sequence. The YopM sequence shares some significant homology with the von Willebrand factor- and thrombin-binding domain of the alpha chain of human platelet membrane glycoprotein Ib. These findings suggested a testable hypothesis for the function of YopM.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics Base Sequence Blotting, Western Cloning, Molecular Escherichia coli/genetics Genes Genes, Bacterial Humans Molecular Sequence Data Plasmids Platelet Membrane Glycoproteins/genetics Restriction Mapping Sequence Homology, Nucleic Acid Yersinia pestis/genetics
Chemicals
Bacterial Outer Membrane Proteins Platelet Membrane Glycoproteins yopM protein, Yersinia
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Leung K Y
Department of Microbiology and Immunology, Chandler Medical Center, University of Kentucky, Lexington 40536.
Straley S C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.9.4623-4632.1989
Published
1989-09-00
Pages
4623-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210260
Subset
IM
Grants
NIAID NIH HHS · AI21017 · United States
Databases
GENBANK
M25810
External Links
PubMed source DOI: 10.1128/jb.171.9.4623-4632.1989 PMC Full Text View journal details
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