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PMID: 2670913 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of the structural gene for alkaline elastase YaB, a new subtilisin produced by an alkalophilic Bacillus strain.

Journal of bacteriology ·Vol. 171 ·No. 9 ·1989-09-00 ·Pages 5232-6

Kaneko R, Koyama N, Tsai YC, Juang RY, Yoda K, Yamasaki M

Abstract

Alkaline elastase YaB is an extracellular serine protease of the alkalophilic Bacillus strain YaB. We cloned the structural gene, ale, and determined the nucleotide sequence. The mature enzyme (268 amino acids) was preceded by a putative signal sequence and a prosequence (27 and 83 amino acids, respectively). The mature enzyme was 55% homologous to subtilisin BPN'. Almost all the positively charged residues are predicted to be on the surface of the molecule, which would facilitate binding to elastin. The P1 substrate site-related sequences differed between alkaline elastase YaB and subtilisin BPN'.

MeSH Terms

Amino Acid Sequence Bacillus/enzymology,genetics Base Sequence Cloning, Molecular Genes Genes, Bacterial Genetic Vectors Hydrogen-Ion Concentration Molecular Sequence Data Plasmids Sequence Homology, Nucleic Acid Subtilisins/genetics

Chemicals

Subtilisins alkaline elastase YaB

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kaneko R

Department of Agricultural Chemistry, University of Tokyo, Japan.

Koyama N

Tsai Y C

Juang R Y

Yoda K

Yamasaki M

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.171.9.5232-5236.1989

Published

1989-09-00

Pages

5232-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC210351

Subset

Databases

GENBANK

M28537

External Links

PubMed source DOI: 10.1128/jb.171.9.5232-5236.1989 PMC Full Text View journal details

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Molecular cloning of the structural gene for alkaline elastase YaB, a new subtilisin produced by an alkalophilic Bacillus strain.

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