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PMID: 2671021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Staphylococcus aureus exopolysaccharide in vivo demonstrated by immunomagnetic separation and electron microscopy.

Journal of clinical microbiology ·Vol. 27 ·No. 7 ·1989-07-00 ·Pages 1631-5

Johne B, Jarp J, Haaheim LR

Abstract

Staphylococcus aureus strains were separated from mastitis milk samples without cultivation by using monodisperse magnetic polymer particles coated with polyclonal antiserum against an encapsulated S. aureus strain. Exopolysaccharide was verified by transmission electron microscopy and the serum soft-agar culture technique. Capsular polysaccharide was found on virtually all clinical isolates. Surface protein A and S. aureus-specific cell wall components were masked when the strains were cultured on an exopolysaccharide-promoting medium. Masking of surface determinants was dependent on their concentration on the bacterial surface as well as on exopolysaccharide abundance. The polysaccharide layer on in vivo bacteria was reduced markedly after just one transfer from milk to blood agar plates but was reexpressed after culturing was done on a capsule-generating medium.

MeSH Terms
Animals Cattle Culture Media Fluorescent Antibody Technique Mastitis, Bovine/microbiology Microscopy, Electron Microspheres Milk/microbiology Polysaccharides, Bacterial/analysis Staphylococcus aureus/analysis,ultrastructure
Chemicals
Culture Media Polysaccharides, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Johne B
National Veterinary Institute, Oslo, Norway.
Jarp J
Haaheim L R
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27 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.27.7.1631-1635.1989
Published
1989-07-00
Pages
1631-5
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC267628
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.27.7.1631-1635.1989 PMC Full Text View journal details
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