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PMID: 2672619 Published · ppublish English Journal Article Review

In vitro amplification techniques for the detection of nucleic acids: new tools for the diagnostic laboratory.

The Yale journal of biology and medicine ·Vol. 62 ·No. 2 ·1989-00-00 ·Pages 159-71

Persing DH, Landry ML

Abstract

The acceptance of nucleic acid probes as diagnostic tools for the clinical laboratory has been hampered by a number of factors, including laborious techniques and limited sensitivity. The focus of this review is on the recent development of amplification techniques to enhance the signal generated by nucleic acid-based detection systems. Three general areas are discussed: (1) amplification of target sequences using the polymerase chain reaction or the transcript amplification system, (2) amplification of the probe sequences using Q beta replicase, and (3) amplification of probe-generated signals with compound or "Christmas tree" probes. The hope of these new technologies is to simplify yet improve on the sensitivity of nucleic acid-based tests to enable them to attain a more prominent place in the diagnostic repertoire of the clinical laboratory.

MeSH Terms
Animals DNA, Viral/analysis Humans Molecular Biology/methods Nucleic Acid Amplification Techniques Q beta Replicase/metabolism RNA, Viral/analysis Virus Diseases/diagnosis
Chemicals
DNA, Viral RNA, Viral Q beta Replicase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Persing D H
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
Landry M L
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Article Info
Journal
The Yale journal of biology and medicine
Abbr.
Yale J Biol Med
ISSN
0044-0086
Published
1989-00-00
Pages
159-71
Language
English
Region
United States
NLM ID
0417414
PMCID
PMC2589213
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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