Binding of the Escherichia coli cyclic AMP receptor protein to DNA fragments containing consensus nucleotide sequences.
Binding of the Escherichia coli CRP protein to DNA fragments carrying nucleotide sequences closely corresponding to the consensus is very tight with a dissociation time of over 2 h in our conditions. The concentration of cyclic AMP required for this binding is below the physiological range of intracellular cyclic AMP concentrations. Changes in nucleotide sequence at positions that are not well-conserved between different naturally-occurring CRP sites allow a more rapid dissociation of CRP-DNA complexes. There is an inverse correlation between the stability of CRP binding to sites in vitro and the repression by glucose of expression dependent on these sites in vivo: expression that is dependent on the tighter binding sites cannot be repressed by the inclusion of glucose in the growth medium.
20 references, click to expand
-
Cyclic AMP receptor protein: role in transcription activation.Science. 1984 May 25;224(4651):831-8 PMID: 6372090
-
The locus of sequence-directed and protein-induced DNA bending.Nature. 1984 Apr 5-11;308(5959):509-13 PMID: 6323997
-
Deletion analysis of the CAP-cAMP binding site of the Escherichia coli lactose promoter.Nucleic Acids Res. 1984 Jul 11;12(13):5449-64 PMID: 6087287
-
Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.Nature. 1984 Sep 20-26;311(5983):232-5 PMID: 6090927
-
Molecular basis of DNA sequence recognition by the catabolite gene activator protein: detailed inferences from three mutations that alter DNA sequence specificity.Proc Natl Acad Sci U S A. 1984 Dec;81(23):7274-8 PMID: 6390433
-
The DNA binding domain and bending angle of E. coli CAP protein.Cell. 1986 Dec 26;47(6):995-1005 PMID: 3536129
-
Probing the sequence-specific interaction of the cyclic AMP receptor protein with DNA by site-directed mutagenesis.Biochem J. 1987 Mar 15;242(3):645-53 PMID: 3109398
-
Electrostatic calculations and model-building suggest that DNA bound to CAP is sharply bent.Proteins. 1987;2(4):283-9 PMID: 2834718
-
DNA sequence determinants of CAP-induced bending and protein binding affinity.Nature. 1988 Jun 30;333(6176):824-9 PMID: 2838756
-
DNA twisting and the affinity of bacteriophage 434 operator for bacteriophage 434 repressor.Proc Natl Acad Sci U S A. 1988 Jul;85(13):4633-7 PMID: 3387430
-
Selection of DNA binding sites by regulatory proteins. II. The binding specificity of cyclic AMP receptor protein to recognition sites.J Mol Biol. 1988 Apr 20;200(4):709-23 PMID: 3045325
-
Alterations in the binding site of the cyclic AMP receptor protein at the Escherichia coli galactose operon regulatory region.Biochem J. 1988 Aug 1;253(3):809-18 PMID: 2845937
-
Empirical estimation of protein-induced DNA bending angles: applications to lambda site-specific recombination complexes.Nucleic Acids Res. 1988 Oct 25;16(20):9687-705 PMID: 2972993
-
Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences.Proc Natl Acad Sci U S A. 1988 Nov;85(21):7947-51 PMID: 3186699
-
Mutational analysis of the nucleotide sequence at the FNR-dependent nirB promoter in Escherichia coli.Nucleic Acids Res. 1989 Jan 11;17(1):135-45 PMID: 2536149
-
Comparison of the binding sites for the Escherichia coli cAMP receptor protein at the lactose and galactose promoters.EMBO J. 1983;2(2):217-22 PMID: 11894929
-
Multiple regulation of the activity of adenylate cyclase in Escherichia coli.Mol Gen Genet. 1982;185(2):262-8 PMID: 6283317
-
Deletion mutagenesis of the Escherichia coli galactose operon promoter region.J Mol Biol. 1983 Jun 25;167(2):259-74 PMID: 6408264
-
On the different binding affinities of CRP at the lac, gal and malT promoter regions.Nucleic Acids Res. 1983 Nov 25;11(22):7833-52 PMID: 6316274
-
Model of specific complex between catabolite gene activator protein and B-DNA suggested by electrostatic complementarity.Proc Natl Acad Sci U S A. 1984 Jul;81(13):3973-7 PMID: 6377305
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