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PMID: 2674945 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Salmonella typhimurium phoP virulence gene is a transcriptional regulator.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 18 ·1989-09-00 ·Pages 7077-81

Groisman EA, Chiao E, Lipps CJ, Heffron F

Abstract

Salmonella typhimurium is a facultative intracellular pathogen capable of surviving within host phagocytic cells. Salmonella strains carrying phoP mutations are avirulent, unable to survive in macrophages, and extremely sensitive to peptides having antimicrobial activity such as the host-derived defensins. We present here the DNA sequence of the phoP gene and show that the deduced amino acid sequence of phoP has extensive homology with the Escherichia coli transcriptional regulators PhoB and OmpR, which control the expression of loci in response to different environmental stimuli. The psiD locus, which is regulated by phosphate availability, was found to be under the control of the phoP gene product. Sequences homologous to phoP were found in several Gram-negative species and in the yeast Saccharomyces cerevisiae.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Genes, Regulator Genotype Molecular Sequence Data Plasmids Restriction Mapping Salmonella typhimurium/genetics,pathogenicity Sequence Homology, Nucleic Acid Transcription, Genetic Virulence
Chemicals
Bacterial Proteins DNA, Bacterial PhoP protein, Bacteria
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Groisman E A
Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, CA 92037.
Chiao E
Lipps C J
Heffron F
References (31)
31 references, click to expand
  1. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages.
    J Mol Biol. 1975 Aug 5;96(2):307-16 PMID: 1100846
  2. A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli.
    Biochem Biophys Res Commun. 1973 Oct 15;54(4):1451-9 PMID: 4585292
  3. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  4. Regulation of nonspecific acid phosphatase in Salmonella: phoN and phoP genes.
    J Bacteriol. 1979 Apr;138(1):155-61 PMID: 374361
  5. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  6. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  7. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  8. A statistical technique for predicting membrane protein structure.
    Biochim Biophys Acta. 1985 Apr 29;828(3):351-61 PMID: 3986213
  9. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  10. Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins.
    J Bacteriol. 1985 Nov;164(2):578-84 PMID: 2997120
  11. Phosphate starvation regulon of Salmonella typhimurium.
    J Bacteriol. 1986 May;166(2):666-9 PMID: 3009420
  12. Pleiotropic mutations in appR reduce pH 2.5 acid phosphatase expression and restore succinate utilisation in CRP-deficient strains of Escherichia coli.
    Mol Gen Genet. 1986 Feb;202(2):257-64 PMID: 3517593
  13. Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent.
    Proc Natl Acad Sci U S A. 1986 Jul;83(14):5189-93 PMID: 3523484
  14. Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon.
    J Bacteriol. 1986 Oct;168(1):294-302 PMID: 3531171
  15. Characterization by deletion and localized mutagenesis in vitro of the promoter region of the Escherichia coli ompC gene and importance of the upstream DNA domain in positive regulation by the OmpR protein.
    J Bacteriol. 1986 Oct;168(1):86-95 PMID: 3531187
  16. Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12.
    J Mol Biol. 1986 Jul 5;190(1):37-44 PMID: 3537313
  17. Cholera toxin transcriptional activator toxR is a transmembrane DNA binding protein.
    Cell. 1987 Jan 30;48(2):271-9 PMID: 3802195
  18. Nucleotide sequence of the phoR gene, a regulatory gene for the phosphate regulon of Escherichia coli.
    J Mol Biol. 1986 Dec 5;192(3):549-56 PMID: 3550103
  19. Conserved domains in bacterial regulatory proteins that respond to environmental stimuli.
    Cell. 1987 Jun 5;49(5):579-81 PMID: 3555840
  20. Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic.
    Infect Immun. 1987 Dec;55(12):3035-43 PMID: 3316029
  21. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  22. Regulation of the phosphate regulon of Escherichia coli. Activation of pstS transcription by PhoB protein in vitro.
    J Mol Biol. 1988 Sep 5;203(1):85-95 PMID: 3054125
  23. Function of a bacterial activator protein that binds to transcriptional enhancers.
    Science. 1989 Feb 3;243(4891):629-35 PMID: 2563595
  24. Coordinate regulation and sensory transduction in the control of bacterial virulence.
    Science. 1989 Feb 17;243(4893):916-22 PMID: 2537530
  25. Phosphatase activity is a constant feature of all isolates of all major species of the family Enterobacteriaceae.
    J Clin Microbiol. 1988 Dec;26(12):2637-41 PMID: 2466048
  26. A Salmonella locus that controls resistance to microbicidal proteins from phagocytic cells.
    Science. 1989 Feb 24;243(4894 Pt 1):1059-62 PMID: 2646710
  27. Linkage map of Salmonella typhimurium, edition VII.
    Microbiol Rev. 1988 Dec;52(4):485-532 PMID: 3070321
  28. A two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulence.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):5054-8 PMID: 2544889
  29. Reversible phosphorylation of an enhancer binding protein regulates the transcription of bacterial nitrogen utilization genes.
    Trends Biochem Sci. 1988 Dec;13(12):475-9 PMID: 2908087
  30. Expression and localization of Escherichia coli alkaline phosphatase synthesized in Salmonella typhimurium cytoplasm.
    J Bacteriol. 1968 Nov;96(5):1601-5 PMID: 4882017
  31. Regulation of two phosphatases and a cyclic phosphodiesterase of Salmonella typhimurium.
    J Bacteriol. 1977 Apr;130(1):420-8 PMID: 192713
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.18.7077
Published
1989-09-00
Pages
7077-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297997
Subset
IM
Grants
NIAID NIH HHS · AI22933 · United States
Databases
GENBANK
M25241
External Links
PubMed source DOI: 10.1073/pnas.86.18.7077 PMC Full Text View journal details
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