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PMID: 268628 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nerve growth factor receptors: identification of distinct classes in plasma membranes and nuclei of embryonic dorsal root neurons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 2785-9

Andres RY, Jeng I, Bradshaw RA

Abstract

Two classes of receptors for 125I-labeled nerve growth factor in chick embryonic dorsal root neurons have been observed. One type is associated with the plasma membrane (or microsomal fraction) and can be completely solubilized by Triton X-100. These receptors display the nonsaturable binding isotherms and curvilinear Scatchard plots previously reported for nerve growth factor receptors in whole cells. The second class of binding sites is located in the nucleus, firmly bound to chromatin. These receptors are not solubilized by detergent, show saturable binding, and yield linear Scatchard plots of the type associated with a single class of binding sites of high affinity. The presence of the two receptor types suggests a bimodal mechanism of action for nerve growth factor.

MeSH Terms
Animals Cell Membrane/metabolism Cell Nucleus/metabolism Chick Embryo Ganglia, Autonomic/metabolism,ultrastructure Kinetics Mice Nerve Growth Factors/metabolism Neurons/ultrastructure Polyethylene Glycols Receptors, Drug/isolation & purification,metabolism Solubility
Chemicals
Nerve Growth Factors Receptors, Drug Polyethylene Glycols
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Andres R Y
Jeng I
Bradshaw R A
References (31)
31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.7.2785
Published
1977-07-00
Pages
2785-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431290
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.7.2785 PMC Full Text View journal details
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