Pulsatile delivery of a leucine supplement during long-term continuous enteral feeding enhances lean growth in term neonatal pigs.

Neonatal pigs are used as a model to study and optimize the clinical treatment of infants who are unable to maintain oral feeding. Using this model, we have previously shown that pulsatile administration of leucine during continuous feeding over 24 h via orogastric tube enhanced protein synthesis in skeletal muscle compared to continuous feeding alone. To determine the long-term effects of leucine pulses, neonatal piglets (n=11-12/group) were continuously fed formula via orogastric tube for 21 d with an additional parenteral infusion of either leucine (800 μmol·kg·h, CON+LEU) or alanine (CON+ALA) for 1 h every 4 h. The results show that body and muscle weights and lean gain were ~25% greater and fat gain was 48% lower in CON+LEU than CON+ALA; weights of other tissues were unaffected by treatment. Fractional protein synthesis rates in longissimus dorsi, gastrocnemius, and soleus muscles were ~30% higher in CON+LEU compared to CON+ALA and were associated with decreased Deptor abundance and increased mTORC1, mTORC2, 4EBP1 and S6K1 phosphorylation, SNAT2 abundance, and association of eIF4E with eIF4G and RagC with mTOR. There were no treatment effects on PKB, eIF2α, eEF2, or PRAS40 phosphorylation, Rheb, SLC38A9, v-ATPase, LAMTOR1, LAMTOR2, RagA, RagC, and LAT1 abundance, the proportion of polysomes to nonpolysomes, or the proportion of mRNAs encoding rpS4 or rpS8 associated with polysomes. Our results demonstrate that pulsatile delivery of a leucine supplement during 21 d of continuous enteral feeding enhances lean growth by stimulating the mTORC1-dependent translation initiation pathway leading to protein synthesis in skeletal muscle of neonates.