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PMID: 2689860 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The HML mating-type cassette of Saccharomyces cerevisiae is regulated by two separate but functionally equivalent silencers.

Molecular and cellular biology ·Vol. 9 ·No. 11 ·1989-11-00 ·Pages 4621-30

Mahoney DJ, Broach JR

Abstract

Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, most likely mediated through two cis-acting sites located on opposite sides of the locus. We showed that deletion of either of these two cis-acting sites from the chromosome did not yield any detectable derepression of HML, while deletion of both sites yielded full expression of the locus. In addition, each of these sites was capable of exerting repression of heterologous genes inserted in their vicinity. Thus, HML expression is regulated by two independent silencers, each fully competent for maintaining repression. This situation was distinct from the organization of the other silent locus, HMR, at which a single silencer served as the predominant repressor of expression. Examination of identifiable domains and binding sites within the HML silencers suggested that silencing activity can be achieved by a variety of combinations of various functional domains.

MeSH Terms
Chromosomes, Fungal Gene Expression Regulation, Fungal Genes, Fungal Genes, Mating Type, Fungal Genes, Regulator RNA, Fungal/analysis RNA, Messenger/analysis Restriction Mapping Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
RNA, Fungal RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mahoney D J
Department of Biology, Princeton University, New Jersey 08544.
Broach J R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.11.4621-4630.1989
Published
1989-11-00
Pages
4621-30
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363608
Subset
IM
Grants
NIGMS NIH HHS · GM34545 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.11.4621-4630.1989 PMC Full Text View journal details
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