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PMID: 2690070 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Ribonucleoprotein and protein factors bind to an H-DNA-forming c-myc DNA element: possible regulators of the c-myc gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 24 ·1989-12-00 ·Pages 9682-6

Davis TL, Firulli AB, Kinniburgh AJ

Abstract

We have located a positive, cis-acting DNA sequence element within the 5' flanking DNA of the c-myc gene (-125 base pairs). This DNA sequence element has a large purine-pyrimidine strand asymmetry and can assume the H-DNA conformation. A factor with the properties of a ribonucleoprotein (RNP) interacts with this DNA region. The interaction of the c-myc DNA sequence element and the RNP involves an RNase H-sensitive mechanism and, therefore, may involve an RNA.DNA hybrid. In addition, a protein factor(s) binds to this DNA sequence element. DNA footprinting and mutant oligonucleotide binding/competition assays implicate a punctate, poly(G.C) recognition/binding sequence for the RNP factor, whereas the major protein factor requires two ACCCT sequence motifs for maximal binding. These results suggest that RNP and protein factors act as positive transcriptional regulators of the c-myc gene, perhaps by altering DNA topology.

MeSH Terms
Base Sequence Cell Line Cloning, Molecular DNA, Neoplasm/genetics,metabolism DNA-Binding Proteins/metabolism Humans Molecular Sequence Data Nuclear Proteins/metabolism Protein Binding Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-myc Proto-Oncogenes Restriction Mapping Ribonucleoproteins/metabolism Transcription, Genetic Transfection
Chemicals
DNA, Neoplasm DNA-Binding Proteins Nuclear Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc Ribonucleoproteins Protein-Tyrosine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Davis T L
Department of Human Genetics, Roswell Park Memorial Institute, Buffalo, NY 14263.
Firulli A B
Kinniburgh A J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.24.9682
Published
1989-12-00
Pages
9682-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298565
Subset
IM
Grants
NCI NIH HHS · CA 43661 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.24.9682 PMC Full Text View journal details
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