Binding of mouse interferon to polynucleotides.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 9 ·1977-09-00 ·Pages 3787-90

De Maeyer-Guignard J, Thang MN, De Maeyer E

Abstract

The polynucleotides poly(I) and poly(U), and to a lesser extent poly(G), are capable of desorbing mouse interferon from blue dextran-Sepharose columns, whereas poly(A) andpoly(C) are without effect. When covalently bound to agarose, poly(I), poly(U), and also poly(A) act as potent ligands for purification of interferon by affinity chromatography. Furthermore, poly(I) and poly(U) confer a significant degree of protection to interferon against thermal denaturation. Taken together, these observations point to a direct interaction of interferon with these polymers and suggest that interferon molecules have a polynucleotide attachment site. Possible implications for the concept of interferon induction are discussed.

MeSH Terms

Cell Line Chemical Phenomena Chemistry Interferons Kinetics Newcastle disease virus Polyribonucleotides Structure-Activity Relationship

Chemicals

Polyribonucleotides Interferons

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

De Maeyer-Guignard J

Thang M N

De Maeyer E

References (12)

12 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.9.3787

Published

1977-09-00

Pages

3787-90

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431731

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.9.3787 PMC Full Text View journal details

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Binding of mouse interferon to polynucleotides.

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