Abstract
During mouse early embryogenesis, blastomeres increase in number by the morula stage. Among them, the outer cells are polarized and differentiated into trophectoderm (TE), while the inner cells remain unpolarized and give rise to inner cell mass (ICM). TE provides an important liquid environment for ICM development. In spite of extensive research, the molecular mechanisms underlying TE formation are still obscure. In order to investigate the roles of estrogen receptor α (ERα) in this course, mouse 8-cell embryos were collected and cultured in media containing ERα specific antagonist MPP and/or agonist PPT. The results indicated that MPP treatment inhibits blastocyst formation in a dose-dependent manner, while PPT, at proper concentration, promotes the cavitation ratio of mouse embryos. Immunofluorescence staining results showed that MPP significantly decreased the nuclear expression of CDX2 in morula, but no significant changes of OCT4 were observed. Moreover, after MPP treatment, the expression levels of the genes related to TE specification, Tead4, Gata3 and Cdx2, were significantly reduced. Overall, these results indicated that ERα might affect mouse embryo cavitation by regulating TE lineage differentiation.
Keywords
blastocyst
differentiation
estrogen receptor alpha
morula
trophectoderm
MeSH 主题词
1-Methyl-4-phenylpyridinium/pharmacology
Animals
Base Sequence
Blastocyst Inner Cell Mass/cytology,metabolism
Blastomeres/cytology,metabolism
CDX2 Transcription Factor/genetics
Cell Differentiation
Cell Lineage
DNA-Binding Proteins/genetics
Ectoderm/cytology,embryology,metabolism
Estrogen Receptor alpha/agonists,antagonists & inhibitors,metabolism
Female
GATA3 Transcription Factor/genetics
Gene Expression Regulation, Developmental/drug effects
Male
Mice
Microscopy, Confocal
Muscle Proteins/genetics
Octamer Transcription Factor-3/genetics
Phenols/pharmacology
Pyrazoles/pharmacology
Reverse Transcriptase Polymerase Chain Reaction
TEA Domain Transcription Factors
Transcription Factors/genetics
Trophoblasts/cytology,metabolism
化学物质
CDX2 Transcription Factor
Cdx2 protein, mouse
DNA-Binding Proteins
Estrogen Receptor alpha
GATA3 Transcription Factor
Gata3 protein, mouse
Muscle Proteins
Octamer Transcription Factor-3
Phenols
Pou5f1 protein, mouse
Pyrazoles
TEA Domain Transcription Factors
Tead4 protein, mouse
Transcription Factors
4,4',4''-(4-propyl-((1)H)-pyrazole-1,3,5-triyl) tris-phenol
1-Methyl-4-phenylpyridinium
作者与单位
共 9 位作者，点击展开单位 / ORCID
Cheng Xiaoxiang
Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
Xu Songhua
Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
Song Chanchan
Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
He Lin
Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
Lian Xiuli
Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
Liu Yue
Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
Wei Jianen
Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China. | Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
Pang Lili
Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
Wang Shie
Cellular and Developmental Engineering Center, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China. | Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350108, China.
