Chemical basis for brain-specific serine transfer RNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 10 ·1977-10-00 ·Pages 4243-7

Rogg H, Müller P, Keith G, Staehelin M

Abstract

Serine tRNA from rat brain can be resolved into six isoaccepting species. Three of these species show the same chromatographic behavior as the seryl tRNAs from other rat organs, whereas the remaining species appear to be specific for brain. The isoacceptor tRNAs were purified to homogeneity by chromatography on benzoylated DEAE-cellulose followed by reversed-phase chromatography. We found that the additional species of serine tRNA in brain differ from their counterparts derived from other rat organs by a lack of a specific guanosine ribose-methylation in the dihydrouridine loop. In addition, when total liver tRNA was compared with total brain tRNA, the same degree of undermethylation with respect to 2'-O-methylguanosine was found as a general phenomenon.

MeSH Terms

Animals Brain Chemistry Chemical Phenomena Chemistry In Vitro Techniques Liver/analysis Nucleosides/analysis Organ Specificity RNA, Transfer/isolation & purification,metabolism Rats Ribose/metabolism Serine/metabolism Time Factors

Chemicals

Nucleosides Serine Ribose RNA, Transfer

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rogg H

Müller P

Keith G

Staehelin M

References (26)

26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.10.4243

Published

1977-10-00

Pages

4243-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431915

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.10.4243 PMC Full Text View journal details

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