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PMID: 270702 Published · ppublish English Case Reports Journal Article Research Support, U.S. Gov't, P.H.S.

Detection of antibody to autologous human leukemia cells by immune adherence assays.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 10 ·1977-10-00 ·Pages 4587-90

Garrett TJ, Takahashi T, Clarkson BD, Old LJ

Abstract

The sera of 21 adult patients with acute leukemia were studied for the presence of antibody reacting with surface antigens of autologous leukemia cells. Sequential serum samples were obtained from patients and were tested on cryopreserved leukemia cells in immune adherence assays. Three patients showed autologous serum reactivity and the serum of one of them was analyzed in detail. This antibody reacted with autologous acute lymphocytic leukemia cells but not with autologous cells obtained from peripheral blood, bone marrow, or spleen during clinical remission. In absorption tests, the antigen could not be detected on normal autologous or allogeneic blood lymphocytes, lymphoblastoid lines of T- or B-cell origin, or cells infected with simian sarcoma virus, baboon C-type virus, or Mason-Pfizer virus. Leukemia cells from two other patients with acute lymphocytic leukemia and one patient with acute nonlymphocytic leukemia absorbed specific reactivity. These studies indicate that certain acute leukemia cells express a common antigen that elicits a humoral immune response in the autologous host.

MeSH Terms

Acute Disease Adult Antibodies, Neoplasm/analysis Cell Line Humans Immune Adherence Reaction Leukemia/immunology Leukemia, Lymphoid/immunology Male Time Factors

Chemicals

Antibodies, Neoplasm

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Garrett T J

Takahashi T

Clarkson B D

Old L J

References (9)

9 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.10.4587

Published

1977-10-00

Pages

4587-90

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431991

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.10.4587 PMC Full Text View journal details

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