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PMID: 270711 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Complete amino acid sequence of rabbit muscle glycogen phosphorylase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 11 ·1977-11-00 ·Pages 4762-6

Titani K, Koide A, Hermann J, Ericsson LH, Kumar S, Wade RD, Walsh KA, Neurath H, Fischer EH

Abstract

The sequence of the 841 amino acid residues in each subunit (molecular weight 97,412) of rabbit muscle glycogen phosphorylase b (1,4-alpha-D-glucan:orthophosphate alpha-glucosyltransferase; EC 2.4.1.1) has been determined. The general strategy was based on limited proteolysis of native phosphorylase b by subtilisin BPN', yielding two large segments (light and heavy) which were fragmented by cleavage at methyonyl-, asparaginyl-glycine, and aspartyl-proline bonds. Analysis of two cyanogen bromide fragments (CB14 and CB17) isolated from the intact molecule yielded the overlap between the light and heavy fragments and the remainder of the sequence. The residues involved in the covalent and allosteric control of the enzyme, and in the binding of the cofactor pyridoxal 5'-phosphate, were identified as serine-14, tyrosine-155, and lysine-679, respectively.

MeSH Terms

Amino Acid Sequence Animals Chemical Phenomena Chemistry Cyanogen Bromide Hydrolysis Muscles/enzymology Peptide Fragments/analysis Phosphorylases Rabbits

Chemicals

Peptide Fragments Phosphorylases Cyanogen Bromide

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Titani K

Koide A

Hermann J

Ericsson L H

Kumar S

Wade R D

Walsh K A

Neurath H

Fischer E H

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.11.4762

Published

1977-11-00

Pages

4762-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432035

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.11.4762 PMC Full Text View journal details

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Complete amino acid sequence of rabbit muscle glycogen phosphorylase.

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