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PMID: 270712 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

HeLa cell poly(A)- mRNA codes for a subset of poly(A)+ mRNA-directed proteins with an actin as a major product.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 11 ·1977-11-00 ·Pages 4801-5

Kaufmann Y, Milcarek C, Berissi H, Penman S

Abstract

Poly(A)+ and poly(A)- mRNA from HeLa cells were separated and translated in heterologous messenger-dependent protein synthesizing systems. Two-dimensional electrophoretic analysis revealed three classes of polypeptides. At the level of detectability in the electropherograms, a small number (about 10) of proteins were detected only among the poly(A)- mRNA products, a larger number (about 40) were produced by both poly(A)- and poly(A)+ mRNA, and a large number of polypeptides were found exclusively in the poly(A)+ mRNA products. The major product of both poly(A)+ and poly(A)- mRNA was shown to be the beta form of actin.Previous cross hybridization measurements suggested little homology between poly(A)+ and poly(A)- mRNA populations. In view of the apparent identity of many poly(A)- products with those of poly(A)+, the homology between poly(A)+ and poly(A)- mRNA sequences was examined in greater detail. cDNA complementary to only the most abundant poly(A)+ message sequences was prepared. About 10% of this cDNA hybridized to abundant sequences in the poly(A)- fraction. This corresponded to only 2% of the total mass of poly(A)+ mRNA and accounted for the failure to detect cross hybridization in previous experiments. Thus, a small number of poly(A)+ sequences appear to be present in relatively high concentration in poly(A)- mRNA as evidenced by both the translation products and the cross hybridization results.

MeSH Terms
Actins/biosynthesis,genetics Base Sequence DNA/biosynthesis Electrophoresis, Polyacrylamide Gel Genetic Code HeLa Cells Humans Poly A/biosynthesis,genetics Protein Biosynthesis RNA, Messenger/genetics,metabolism
Chemicals
Actins RNA, Messenger Poly A DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kaufmann Y
Milcarek C
Berissi H
Penman S
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.11.4801
Published
1977-11-00
Pages
4801-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432043
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.11.4801 PMC Full Text View journal details
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