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PMID: 2710121 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical linkage of mouse lambda genes by pulsed-field gel electrophoresis suggests that the rearrangement process favors proximate target sequences.

Molecular and cellular biology ·Vol. 9 ·No. 2 ·1989-02-00 ·Pages 711-8

Storb U, Haasch D, Arp B, Sanchez P, Cazenave PA, Miller J

Abstract

The first complete map of a mammalian immunoglobulin gene locus is presented. Mouse lambda genes were mapped by pulsed-field gel electrophoresis. The gene order is V2-Vx-C2-C4-V1-C3-C1. The distance between V2 or Vx and the C2-C4 cluster is 74 or 55 kilobases (kb), respectively, whereas that between V1 and C3-C1 is only 19 kb; V2 and C3-C1 are at least 190 kb apart. Thus, the distances between the lambda subloci are inversely proportional to their frequencies of rearrangement. The related gene lambda 5 is not within the 500 kb of the lambda locus mapped here.

MeSH Terms
Animals Chromosome Mapping Cloning, Molecular DNA Probes Electrophoresis, Agar Gel Gene Rearrangement, B-Lymphocyte, Light Chain Genetic Linkage Mice Restriction Mapping
Chemicals
DNA Probes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Storb U
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Haasch D
Arp B
Sanchez P
Cazenave P A
Miller J
References (35)
35 references, click to expand
  1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  2. Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation.
    Nature. 1981 Jul 23;292(5821):311-7 PMID: 6166864
  3. Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1485-9 PMID: 418414
  4. Sequences of mouse immunoglobulin light chain genes before and after somatic changes.
    Cell. 1978 Dec;15(4):1133-44 PMID: 103630
  5. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
    J Immunol. 1979 Oct;123(4):1548-50 PMID: 113458
  6. Cellular differentiation, cytidine analogs and DNA methylation.
    Cell. 1980 May;20(1):85-93 PMID: 6156004
  7. Immunoglobulin gene expression in transformed lymphoid cells.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):825-9 PMID: 6402777
  8. A lymphocyte-specific enhancer in the mouse immunoglobulin kappa gene.
    Nature. 1984 Jan 5-11;307(5946):80-2 PMID: 6419128
  9. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
    Cell. 1984 May;37(1):67-75 PMID: 6373014
  10. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.
    Nucleic Acids Res. 1984 Jul 25;12(14):5647-64 PMID: 6379602
  11. Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell lines.
    Nature. 1984 Oct 25-31;311(5988):727-33 PMID: 6092962
  12. A single rearrangement event generates most of the chicken immunoglobulin light chain diversity.
    Cell. 1985 Feb;40(2):283-91 PMID: 3917859
  13. Developmentally controlled expression of immunoglobulin VH genes.
    Science. 1985 Mar 29;227(4694):1597-601 PMID: 3975629
  14. Immunoglobulin genes.
    Annu Rev Immunol. 1983;1:499-528 PMID: 6443559
  15. Lambda chains and genes in inbred mice.
    Annu Rev Immunol. 1985;3:337-65 PMID: 3933530
  16. Introduced T cell receptor variable region gene segments recombine in pre-B cells: evidence that B and T cells use a common recombinase.
    Cell. 1986 Jan 31;44(2):251-9 PMID: 3484682
  17. Organization of four mouse lambda light chain immunoglobulin genes.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3765-9 PMID: 6791160
  18. Physical linkage of the constant region genes for immunoglobulins lambda I and lambda III.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3829-33 PMID: 6267606
  19. Structural alterations in J regions of mouse immunoglobulin lambda genes are associated with differential gene expression.
    Nature. 1982 Feb 4;295(5848):428-30 PMID: 6799835
  20. Evolution of mouse immunoglobulin lambda genes.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4681-5 PMID: 6812053
  21. EcoK restriction during in vitro packaging of coliphage lambda DNA.
    Gene. 1985;39(2-3):313-5 PMID: 3005116
  22. Role of DNA topology in Mu transposition: mechanism of sensing the relative orientation of two DNA segments.
    Cell. 1986 Jun 20;45(6):793-800 PMID: 3011279
  23. Lambda 5, a new light-chain-related locus selectively expressed in pre-B lymphocytes.
    Nature. 1986 Dec 11-17;324(6097):579-82 PMID: 3024017
  24. Organization of the murine Ig-related lambda 5 gene transcribed selectively in pre-B lymphocytes.
    EMBO J. 1987 Jan;6(1):103-7 PMID: 3107979
  25. A new V gene expressed in lambda-2 light chains of the mouse.
    Eur J Immunol. 1987 May;17(5):731-4 PMID: 3034625
  26. Active lambda and kappa antibody gene rearrangement in Abelson murine leukemia virus-transformed pre-B cell lines.
    J Exp Med. 1987 Jun 1;165(6):1655-74 PMID: 3035056
  27. A new variable region in mouse immunoglobulin lambda light chains.
    J Exp Med. 1987 Jul 1;166(1):265-70 PMID: 3110349
  28. A second gene, VpreB in the lambda 5 locus of the mouse, which appears to be selectively expressed in pre-B lymphocytes.
    EMBO J. 1987 Aug;6(8):2267-72 PMID: 3117530
  29. T-cell receptor delta gene rearrangements in early thymocytes.
    Nature. 1987 Dec 24-31;330(6150):722-7 PMID: 2961997
  30. Structure of a third murine immunoglobulin lambda light chain variable region that is expressed in laboratory mice.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9185-8 PMID: 3122215
  31. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
    Nucleic Acids Res. 1988 Feb 25;16(4):1563-75 PMID: 2831502
  32. The order and orientation of mouse lambda-genes explain lambda-rearrangement patterns.
    J Immunol. 1988 Oct 1;141(7):2497-502 PMID: 3139761
  33. Ig lambda-producing B cells do not show feedback inhibition of gene rearrangement.
    J Immunol. 1988 Oct 15;141(8):2771-80 PMID: 3139764
  34. Transverse alternating field electrophoresis (TAFE).
    Biotechniques. 1988 Jan;6(1):68-73 PMID: 3273395
  35. Expression of immunoglobulin and globin genes in B and T lymphocytes and other cells.
    Biochemistry. 1977 Dec 13;16(25):5432-8 PMID: 303524
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.2.711-718.1989
Published
1989-02-00
Pages
711-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362648
Subset
IM
Grants
NIAID NIH HHS · AI24780 · United States
NIGMS NIH HHS · GM38649 · United States
NICHD NIH HHS · HD23089 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.2.711-718.1989 PMC Full Text View journal details
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