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PMID: 2715314 Published · ppublish English Journal Article

Genetic and antigenic characterization of Borrelia coriaceae, putative agent of epizootic bovine abortion.

Journal of clinical microbiology ·Vol. 27 ·No. 3 ·1989-03-00 ·Pages 389-93

LeFebvre RB, Perng GC

Abstract

Borrelia coriaceae was characterized genetically and antigenically by utilizing the following techniques: restriction endonuclease analysis, Southern blotting and genomic hybridization, pulsed-field electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting. The B. coriaceae genome revealed unique and characteristic banding patterns both by agarose gel electrophoresis and by hybridization when compared with several Borrelia burgdorferi isolates. Pulsed-field gel electrophoresis demonstrated several linear plasmids ranging from 65 to 30 kilobase pairs. Cross-reaction with B. burgdorferi antigens ranging from 21 to 26 kilodaltons were demonstrated by immunoblotting with rabbit anti-B. coriaceae antiserum. However, most B. coriaceae antigens were quite distinct when compared with B. burgdorferi and Leptospira interrogans antigens.

MeSH Terms
Abortion, Veterinary/microbiology Animals Antigens, Bacterial/analysis Autoradiography Blotting, Southern Blotting, Western Borrelia/genetics,immunology Cattle Cattle Diseases/microbiology DNA, Bacterial/analysis Deoxyribonuclease HindIII Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Female Humans Nucleic Acid Hybridization Plasmids Pregnancy Restriction Mapping
Chemicals
Antigens, Bacterial DNA, Bacterial Deoxyribonuclease HindIII
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
LeFebvre R B
Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Perng G C
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.27.3.389-393.1989
Published
1989-03-00
Pages
389-93
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC267327
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.27.3.389-393.1989 PMC Full Text View journal details
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