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PMID: 2725508 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Electrophoretic analysis of Histoplasma capsulatum chromosomal DNA.

Molecular and cellular biology ·Vol. 9 ·No. 3 ·1989-03-00 ·Pages 983-7

Steele PE, Carle GF, Kobayashi GS, Medoff G

Abstract

Seven chromosome-sized DNA molecules in the Downs strain of Histoplasma capsulatum were resolved by using chromosome-specific DNA probes in blot hybridizations of contour-clamped homogeneous electric field (CHEF) and field-inversion gel electrophoresis (FIGE) agarose gels. The sizes of the chromosomal DNA bands extended from that of the largest Saccharomyces cerevisiae chromosome to beyond that of the Schizosaccharomyces pombe chromosomes. Under our experimental conditions, the order of the five largest DNA bands was inverted in the FIGE gel relative to the CHEF gel, demonstrating a characteristic of FIGE whereby large DNA molecules may have greater rather than lesser mobility with increasing size. Comparison of the Downs strain with other H. capsulatum strains by CHEF and FIGE analysis revealed considerable variability in band mobility. The resolution of seven chromosome-sized DNA molecules in the Downs strain provides a minimum estimate of the chromosome number.

MeSH Terms
Chromosomes DNA, Fungal/genetics,isolation & purification Electrophoresis, Agar Gel Histoplasma/analysis,genetics Polymorphism, Genetic Species Specificity
Chemicals
DNA, Fungal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Steele P E
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Carle G F
Kobayashi G S
Medoff G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.3.983-987.1989
Published
1989-03-00
Pages
983-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362687
Subset
IM
Grants
NIAID NIH HHS · 5F32AIO7592-02 · United States
NIAID NIH HHS · AI 07015 · United States
NIAID NIH HHS · AI 16228 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.3.983-987.1989 PMC Full Text View journal details
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