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PMID: 2730588 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Properties and fluctuations in vivo of rat liver antizyme inhibitor.

The Biochemical journal ·Vol. 259 ·No. 3 ·1989-05-01 ·Pages 839-45

Murakami Y, Matsufuji S, Nishiyama M, Hayashi S

Abstract

Antizyme inhibitor was highly purified from rat liver by using affinity chromatography. It has some structural resemblance to ornithine decarboxylase (ODC), as judged from Mr, immunoreactivity and reversible binding with antizyme. However, unlike hepatic amounts of ODC and ODC-antizyme complex, that of antizyme inhibitor did not show much fluctuation upon putrescine treatment, whereas it decreased as rapidly as ODC decay in the presence of cycloheximide. These results suggested that antizyme inhibitor is an independent regulatory protein rather than a derivative of ODC. Changes in hepatic amounts of antizyme inhibitor, antizyme and ODC upon feeding suggested that antizyme inhibitor may play a role in ODC regulation by trapping antizyme and thereby suppressing ODC degradation. A monoclonal antibody to rat liver antizyme inhibitor was obtained. This antibody was shown to be utilizable for a simple assay of antizyme-inhibitor activity in tissue extracts.

MeSH Terms
Animals Antibodies, Monoclonal Carrier Proteins Liver/analysis Ornithine Decarboxylase Inhibitors Proteins/analysis,antagonists & inhibitors,physiology Rats Rats, Inbred Strains
Chemicals
Antibodies, Monoclonal Carrier Proteins Ornithine Decarboxylase Inhibitors Proteins ornithine decarboxylase antizyme ornithine decarboxylase antizyme inhibitor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Murakami Y
Department of Nutrition, Jikei University School of Medicine, Tokyo, Japan.
Matsufuji S
Nishiyama M
Hayashi S
References (19)
19 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2590839
Published
1989-05-01
Pages
839-45
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1138593
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2590839 PMC Full Text View journal details
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