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PMID: 273251 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 2 ·1978-02-00 ·Pages 901-4

Hutchings SE, Sato GH

Abstract

HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equal to that of serum-supplemented medium. The omission of any one of the five components results in less than maximal cell growth.

MeSH Terms

Culture Media Growth Substances HeLa Cells Hormones

Chemicals

Culture Media Growth Substances Hormones

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hutchings S E

Sato G H

References (16)

16 references, click to expand

A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS.
Proc Natl Acad Sci U S A. 1955 Jul 15;41(7):432-7 PMID: 16589695
Growth factors for mammalian cells in culture.
J Biol Chem. 1959 Oct;234:2754-8 PMID: 14416837
Nutrition needs of mammalian cells in tissue culture.
Science. 1955 Sep 16;122(3168):501-14 PMID: 13255879
CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.
Proc Natl Acad Sci U S A. 1965 Feb;53:288-93 PMID: 14294058
CHEMICALLY DEFINED MEDIA FOR CULTIVATION OF LONG-TERM CELL STRAINS FROM FOUR MAMMALIAN SPECIES.
Exp Cell Res. 1964 Dec;36:439-74 PMID: 14242231
A hormonally controlled serum factor which stimulates sulfate incorporation by cartilage in vitro.
J Lab Clin Med. 1957 Jun;49(6):825-36 PMID: 13429201
The specific amino acid requirements of a human carcinoma cell (Stain HeLa) in tissue culture.
J Exp Med. 1955 Jul 1;102(1):37-48 PMID: 14392239
Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media.
Exp Cell Res. 1971 Aug;67(2):295-304 PMID: 4328993
Growth response to hormones by a new rat ovary cell line.
Nat New Biol. 1972 Apr 12;236(67):180-1 PMID: 4260653
Pituitary extracts and steroid hormones in the control of 3T3 cell growth.
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702-6 PMID: 4354860
Epidermal growth factor: chemical and biological characterization.
Recent Prog Horm Res. 1974;30(0):533-50 PMID: 4601978
Human somatomedians. Purification and some studies on their biological actions.
Acta Endocrinol Suppl (Copenh). 1973;175:1-35 PMID: 4542208
Replacement of serum by hormones permits growth of cells in a defined medium.
Nature. 1976 Jan 15;259(5539):132-4 PMID: 813153
Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2023-7 PMID: 1064872
Purification of a fibroblast growth factor from bovine pituitary.
J Biol Chem. 1975 Apr 10;250(7):2515-20 PMID: 1168187
The choline and serum protein requirements of mouse fibroblast cells (strain LS) in culture.
J Cell Sci. 1969 Jul;5(1):135-42 PMID: 5353651

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.2.901

Published

1978-02-00

Pages

901-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC411365

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.2.901 PMC Full Text View journal details

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Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.

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