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PMID: 2733691 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes.

Molecular & general genetics : MGG ·Vol. 216 ·No. 1 ·1989-03-00 ·Pages 81-90

Colot V, Bartels D, Thompson R, Flavell R

Abstract

The isolation and characterisation by DNA sequencing of a low molecular weight (LMW) glutenin gene from wheat is described. The deduced protein contains a signal peptide, a central repetitive region rich in proline and glutamine and N and C terminal non-repetitive domains, similar to other prolamins. A detailed comparison of the C terminal domain of 20 prolamin genes enabled us to divide them into 4 families. The LMW glutenin family is distinct from the alpha, beta- and gamma-gliadin families of wheat and is closest to the B hordein genes of barley. This and other comparisons were also used to assess the pattern of genetic variation among prolamin sequences and to provide a molecular basis for the interpretation of prolamin size polymorphism. The 5' flanking fragment of the isolated gene was previously shown to direct endosperm-specific expression of a reporter gene in transgenic tobacco. Evidence is provided that the isolated gene is also active in wheat and its transcription initiation site was determined. Features of the gene which may be relevant to its activity are discussed.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics Glutens/analogs & derivatives,genetics Hordeum/genetics Molecular Sequence Data Molecular Weight Plant Proteins/genetics Plants/genetics Prolamins Triticum/genetics
Chemicals
Plant Proteins Prolamins Glutens DNA glutenin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Colot V
Department of Molecular Genetics, AFRC Institute of Plant Science Research (Cambridge Laboratory), Trumpington, UK.
Bartels D
Thompson R
Flavell R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332234
Published
1989-03-00
Pages
81-90
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332234
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