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PMID: 27338736 Published · ppublish English Journal Article

Mitochondrial membrane potential (MMP) regulates sperm motility.

In vitro cellular & developmental biology. Animal ·Vol. 52 ·No. 9 ·2016-10-00 ·Pages 953-960

Agnihotri SK, Agrawal AK, Hakim BA, Vishwakarma AL, Narender T, Sachan R, Sachdev M

Abstract

Sperm motility is the major decisive factor in determining male fertility. The objective of the present study was to analyse the effect of mitochondrial membrane potential (MMP) on the temporal regulation of sperm motility. Observations were recorded in various rodent species and among differentially motile sperm fractions including swim up and leftover layer of human semen sample using JC-1 stain (a marker of the MMP) through FACS. Swim-up sperms having highest motility showed significantly higher MMP as compared to leftover sperms, which had the least motility. Interestingly, infertile patients with compromised motility showed low MMP as compared to the healthy individuals. Further, as per the time lapse, sperm motility goes down, at the same time, it was observed that MMP also decreases in human as well as in rodent sperms. Treatment of known spermicides on human sperms reduced their motility drastically which in turn also reduced its MMP significantly. Treatment of human sperms with oxidative uncoupler also impeded their motility by reducing MMP, indicating a definitive role on MMP on sperm motility and fertility. Based on the results of the study, MMP can be considered as a potential regulator and indicator of sperm motility and hence could be directly related to male fertility.

Keywords
Infertility Mitochondrial membrane potential Sperm motility Spermatozoa
MeSH Terms
Animals Benzimidazoles/metabolism Carbocyanines/metabolism Carbonyl Cyanide m-Chlorophenyl Hydrazone/analogs & derivatives,pharmacology Detergents/pharmacology Humans Infertility, Male/metabolism Male Membrane Potential, Mitochondrial/drug effects Mice Oxidation-Reduction/drug effects Rats, Sprague-Dawley Sperm Motility/drug effects Spermatocidal Agents/pharmacology Spermatozoa/drug effects,metabolism Time-Lapse Imaging Uncoupling Agents/pharmacology
Chemicals
Benzimidazoles Carbocyanines Detergents Spermatocidal Agents Uncoupling Agents 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine Carbonyl Cyanide m-Chlorophenyl Hydrazone
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Agnihotri Saurabh Kumar
Endocrinology Division, CSIR- Central Drug Research Institute, Lucknow, UP, 226 031, India.
Agrawal Ankit Kumar
Endocrinology Division, CSIR- Central Drug Research Institute, Lucknow, UP, 226 031, India.
Hakim Bilal Ahmad
Endocrinology Division, CSIR- Central Drug Research Institute, Lucknow, UP, 226 031, India.
Vishwakarma A L
Sophisticated Analytical Instrument Facility, CSIR- Central Drug Research Institute, Lucknow, UP, India.
Narender T
Medicinal and Process Chemistry Division, CSIR- Central Drug Research Institute, Lucknow, UP, India.
Sachan Rekha
Department of Obstetrics and Gynaecology, King Georges' Medical University, Lucknow, UP, India.
Sachdev Monika
Endocrinology Division, CSIR- Central Drug Research Institute, Lucknow, UP, 226 031, India. [email protected].
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Article Info
Journal
In vitro cellular & developmental biology. Animal
Abbr.
In Vitro Cell Dev Biol Anim
ISSN
1543-706X
DOI
10.1007/s11626-016-0061-x
Email
[email protected]
Published
2016-10-00
Epub
2016-00-23
Pages
953-960
Language
English
Region
Germany
NLM ID
9418515
Subset
IM
External Links
PubMed source DOI: 10.1007/s11626-016-0061-x View journal details
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