Home LiteratureArticle Details
PMID: 2734310 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A gene encoding the tryptophan synthase beta subunit of Arabidopsis thaliana.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 12 ·1989-06-00 ·Pages 4604-8

Berlyn MB, Last RL, Fink GR

Abstract

The DNA sequence of a tryptophan synthase gene and the flanking 5' and 3' regions has been determined for Arabidopsis thaliana. The sequence encodes only the beta subunit domain, indicating that alpha and beta subunits are specified by separate genes. The gene contains four introns and encodes 470 amino acid residues. The plant amino acid sequence is highly conserved with respect to corresponding microbial sequences. The NH2-terminal amino acid sequence is characteristic of chloroplast transit peptides. Identity of the sequences of the genomic exons and a cDNA clone and the presence of cellular RNA corresponding in size and 5' sequence to the gene indicate that the gene is expressed. Analysis of Arabidopsis genomic DNA suggests the presence of a second gene for the beta subunit.

MeSH Terms
Amino Acid Sequence Base Sequence Genes Macromolecular Substances Molecular Sequence Data Plants/enzymology,genetics Restriction Mapping Sequence Homology, Nucleic Acid Species Specificity Tryptophan Synthase/genetics
Chemicals
Macromolecular Substances Tryptophan Synthase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berlyn M B
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.
Last R L
Fink G R
References (40)
40 references, click to expand
  1. Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase: sequence analysis and manipulation to obtain glyphosate-tolerant plants.
    Mol Gen Genet. 1987 Dec;210(3):437-42 PMID: 3481024
  2. Molecular cloning and DNA sequence of the Arabidopsis thaliana alcohol dehydrogenase gene.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1408-12 PMID: 2937058
  3. Evidence for chain termination by super-suppressible mutants in yeast.
    Genetics. 1968 Dec;60(4):719-33 PMID: 5732414
  4. Host/vector interactions which affect the viability of recombinant phage lambda clones.
    Gene. 1986;49(2):253-62 PMID: 2952553
  5. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  6. Synthesis of tryptophan from chorismate: comparative aspects.
    Methods Enzymol. 1987;142:293-300 PMID: 3298977
  7. The molecular basis of sulfonylurea herbicide resistance in tobacco.
    EMBO J. 1988 May;7(5):1241-8 PMID: 16453837
  8. Enzymic properties of a mutant tryptophan synthase from Neurospora crassa.
    Biochim Biophys Acta. 1972 Sep 19;284(1):324-40 PMID: 4262962
  9. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  10. Structure of the Caulobacter crescentus trpFBA operon.
    J Bacteriol. 1988 Feb;170(2):757-68 PMID: 2828322
  11. Location of three active site residues in the NH2-terminal sequence of the beta 2 subunit tryptophan synthase from Escherichia coli.
    J Biol Chem. 1980 Jan 25;255(2):512-7 PMID: 6985892
  12. The transport of proteins into chloroplasts.
    Annu Rev Biochem. 1986;55:879-912 PMID: 3527053
  13. The complete nucleotide sequence of the tryptophan operon of Escherichia coli.
    Nucleic Acids Res. 1981 Dec 21;9(24):6647-68 PMID: 7038627
  14. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  15. Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium.
    J Mol Biol. 1980 Oct 5;142(4):489-502 PMID: 7007651
  16. How signal sequences maintain cleavage specificity.
    J Mol Biol. 1984 Feb 25;173(2):243-51 PMID: 6423828
  17. Nucleotide sequence of the Bacillus subtilis tryptophan operon.
    Gene. 1985;34(2-3):169-77 PMID: 3924737
  18. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
  19. Nucleotide sequence of the Neurospora crassa trp-3 gene encoding tryptophan synthetase and comparison of the trp-3 polypeptide with its homologs in Saccharomyces cerevisiae and Escherichia coli.
    J Biol Chem. 1989 Mar 5;264(7):3840-8 PMID: 2521855
  20. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  21. Translocation of the precursor of 5-enolpyruvylshikimate-3-phosphate synthase into chloroplasts of higher plants in vitro.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6873-7 PMID: 16593759
  22. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  23. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853-7 PMID: 283395
  24. Partial purification and properties of tryptophan synthase of pea plants.
    Arch Biochem Biophys. 1972 Apr;149(2):402-13 PMID: 4666111
  25. Chloroplast and cytosolic glutamine synthetase are encoded by homologous nuclear genes which are differentially expressed in vivo.
    J Biol Chem. 1988 Jul 15;263(20):9651-7 PMID: 2898472
  26. Neurospora tryptophan synthase. Characterization of the pyridoxal phosphate binding site.
    J Biol Chem. 1988 May 15;263(14):6872-6 PMID: 2966157
  27. Tryptophan-Requiring Mutants of the Plant Arabidopsis thaliana.
    Science. 1988 Apr 15;240(4850):305-10 PMID: 17796738
  28. ON THE SEPARATION OF THE TRYPTOPHAN SYNTHETASE OF ESCHERICHIA COLI INTO TWO PROTEIN COMPONENTS.
    Proc Natl Acad Sci U S A. 1958 Dec 15;44(12):1161-70 PMID: 16590328
  29. Tryptophan synthase from Nicotiana tabacum.
    Biochim Biophys Acta. 1968 Oct 8;167(2):431-43 PMID: 4892516
  30. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  31. Structure, expression, and evolution of the 5-enolpyruvylshikimate-3-phosphate synthase genes of petunia and tomato.
    J Biol Chem. 1988 Mar 25;263(9):4280-7 PMID: 3346248
  32. Purification and some properties of tryptophan synthase inactivase II from yeast.
    Eur J Biochem. 1974 Feb 15;42(1):61-6 PMID: 4598105
  33. L-serine binds to arginine-148 of the beta 2 subunit of Escherichia coli tryptophan synthase.
    Biochemistry. 1983 Jul 19;22(15):3594-603 PMID: 6412746
  34. Production of single-stranded plasmid DNA.
    Methods Enzymol. 1987;153:3-11 PMID: 3323803
  35. Plant intron sequences: evidence for distinct groups of introns.
    Nucleic Acids Res. 1988 Jul 25;16(14B):7159-76 PMID: 3405760
  36. Molecular analysis of the alcohol dehydrogenase 2 (Adh2) gene of maize.
    Nucleic Acids Res. 1985 Feb 11;13(3):727-43 PMID: 2987807
  37. Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa.
    Mol Biol Evol. 1986 May;3(3):191-204 PMID: 3127651
  38. THE EFFECTS OF DELETIONS, POINT MUTATIONS, REVERSIONS AND SUPPRESSOR MUTATIONS ON THE TWO COMPONENTS OF THE TRYPTOPHAN SYNTHETASE OF ESCHERICHIA COLI.
    Proc Natl Acad Sci U S A. 1959 Jul;45(7):1016-26 PMID: 16590470
  39. Yeast gene TRP5: structure, function, regulation.
    J Biol Chem. 1982 Feb 10;257(3):1491-500 PMID: 6276387
  40. Accurate in vitro splicing of two pre-mRNA plant introns in a HeLa cell nuclear extract.
    EMBO J. 1986 Nov;5(11):2749-58 PMID: 2431897
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.12.4604
Published
1989-06-00
Pages
4604-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287319
Subset
IM
Databases
GENBANK
M23872
External Links
PubMed source DOI: 10.1073/pnas.86.12.4604 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2