Complete nucleotide sequence and characterization of a cryptic plasmid from Lactobacillus helveticus subsp. jugurti.

Applied and environmental microbiology ·Vol. 55 ·No. 6 ·1989-06-00 ·Pages 1653-5

Takiguchi R, Hashiba H, Aoyama K, Ishii S

Abstract

A small cryptic plasmid, pLJ1, was isolated from Lactobacillus helveticus subsp. jugurti and was cloned into Escherichia coli HB101 by using pBR329 as a vector. Plasmid pLJ1 was 3,292 base pairs long and had single restriction endonuclease sites for PvuII, KpnI, AvaII, Acci, HindIII, and EcoRI. In a maxicell system, pLJ1 produced a protein of about 41 kilodaltons.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Bacterial/genetics Lactobacillus/genetics Molecular Sequence Data Plasmids Restriction Mapping

Chemicals

DNA, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Takiguchi R

Technical Research Institute, Snow Brand Products Co., Ltd., Saitama, Japan.

Hashiba H

Aoyama K

Ishii S

References (9)

9 references, click to expand

Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication.
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Plasmids, Lactic Acid Production, and N-Acetyl-d-Glucosamine Fermentation in Lactobacillus helveticus subsp. jugurti.
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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.55.6.1653-1655.1989

Published

1989-06-00

Pages

1653-5

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC202923

Subset

External Links

PubMed source DOI: 10.1128/aem.55.6.1653-1655.1989 PMC Full Text View journal details

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Complete nucleotide sequence and characterization of a cryptic plasmid from Lactobacillus helveticus subsp. jugurti.

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