Carbohydrate structure and cell differentitation: unique properties of fucosyl-glycopeptides isolated from embryonal carcinoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 5 ·1978-05-00 ·Pages 2315-9

Muramatsu T, Gachelin G, Nicolas JF, Condamine H, Jakob H, Jacob F

Abstract

From embryonal carcinoma cells labeled with fucose, two main classes of glycopeptide products of Pronase digestion can be distinguished by Sephadex G-50 column chromatography: one eluted near the excluded volume and a smaller one. The large fucosyl-glycopeptides are scarcely present in differentiated cells derived from embryonal carcinoma cells (i.e., fibroblastlike cells, myoblasts, and parietal yolk-sac carcinoma). During in vitro differentiation of embryonal carcinoma cells, these large glycopeptides disappear almost completely. The small glycopeptides were analyzed by paper electrophoresis, concanavalin A-Sepharose affinity chromatography, and digestion with an endoglycosidase. The major components of these glycopeptides from embryonal carcinoma cells appear to be different from complex glycopeptides known to occur in adult cells. The glycopeptide pattern of mouse preimplantation embryos resembles that of embryonal carcinoma cells. These results suggest that the carbohydrate profile changes fundamentally during early stages of mammalian development.

MeSH Terms

Cell Differentiation Cell Line Fucose/metabolism Glycopeptides/metabolism Glycoproteins/metabolism Molecular Weight Neoplasms, Experimental/pathology Teratoma/pathology

Chemicals

Glycopeptides Glycoproteins Fucose

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Muramatsu T

Gachelin G

Nicolas J F

Condamine H

Jakob H

Jacob F

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.5.2315

Published

1978-05-00

Pages

2315-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392543

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.5.2315 PMC Full Text View journal details

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Carbohydrate structure and cell differentitation: unique properties of fucosyl-glycopeptides isolated from embryonal carcinoma cells.

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