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PMID: 2771638 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Analysis of the collagen alpha 1(I) promoter.

Nucleic acids research ·Vol. 17 ·No. 15 ·1989-08-11 ·Pages 6055-64

Brenner DA, Rippe RA, Veloz L

Abstract

The collagen alpha 1(I) gene is regulated at a developmental and tissue specific level. We have previously demonstrated that only 220bp of the promoter region of the collagen alpha 1(I) gene are required for efficient expression in NIH 3T3 cells. DNAse I protection assays demonstrated 4 footprinted segments in the promoter region. Deletional analysis revealed that the 3 most proximal footprints were required for maximal expression. The most proximal footprint contains a CCAAT sequence and a 12bp segment that forms a direct repeat with the preceding footprint. Ligation of the proximal footprint sequence to a heterologous promoter enhanced transcription of the reporter gene. These studies, therefore, identify and characterize elements in the promoter region of the collagen alpha 1(I) gene that interact with DNA binding proteins and are required for efficient expression.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line Collagen/genetics DNA-Binding Proteins/metabolism Deoxyribonuclease I/metabolism Gene Expression Regulation Mice Molecular Sequence Data Plasmids Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Transcription, Genetic Transfection
Chemicals
DNA-Binding Proteins Collagen Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brenner D A
Center for Molecular Genetics, University of California, San Diego 92093.
Rippe R A
Veloz L
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.15.6055
Published
1989-08-11
Pages
6055-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318260
Subset
IM
Grants
NIDDK NIH HHS · DK07202 · United States
External Links
PubMed source DOI: 10.1093/nar/17.15.6055 PMC Full Text View journal details
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