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PMID: 2777375 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Augmentation of the neutrophil response to Naegleria fowleri by tumor necrosis factor alpha.

Infection and immunity ·Vol. 57 ·No. 10 ·1989-10-00 ·Pages 3110-5

Ferrante A

Abstract

Conditioned medium from phytohemagglutinin-stimulated human mononuclear leukocytes, previously shown to activate neutrophils for amoeba killing, was found to contain high levels of tumor necrosis factor alpha (TNF-alpha) by an enzyme-linked immunosorbent assay. The effects of human recombinant TNF-alpha on the response of human neutrophils to the pathogenic free-living amoeba Naegleria fowleri was studied in vitro. The data showed that recombinant human TNF-alpha augmented the neutrophil respiratory burst (assessed by the cytochrome c reduction assay and lucigenin-dependent chemiluminescence assay) in response to amoebae opsonized with human serum. The priming effects of TNF-alpha were transient; marked enhancement was found with short 5- to 30-min preincubations of neutrophils with the cytokine. The enhancement of oxygen radical production was evident with 20 U of TNF-alpha per 10(6) neutrophils and continued to increase with up to 100 U. TNF-alpha also augmented the neutrophil lysosomal enzyme release in response to N. fowleri. The results support previous reports suggesting an important role of neutrophil cytokine activation for effective immunity against free-living amoebae.

MeSH Terms
Adjuvants, Immunologic/pharmacology Adsorption Animals Antibodies, Monoclonal Culture Media Cytoplasmic Granules/metabolism Humans Leukocytes, Mononuclear/immunology,metabolism Naegleria/immunology Neutrophils/immunology,metabolism Oxygen Consumption Phytohemagglutinins Recombinant Proteins/pharmacology Tumor Necrosis Factor-alpha/biosynthesis,immunology,pharmacology
Chemicals
Adjuvants, Immunologic Antibodies, Monoclonal Culture Media Phytohemagglutinins Recombinant Proteins Tumor Necrosis Factor-alpha
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Ferrante A
Department of Immunology, Adelaide Children's Hospital, South Australia.
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39 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.10.3110-3115.1989
Published
1989-10-00
Pages
3110-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260777
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.10.3110-3115.1989 PMC Full Text View journal details
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