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PMID: 2779520 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of a gene cluster from Bacillus stearothermophilus comprising infC, rpmI and rplT.

Molecular & general genetics : MGG ·Vol. 218 ·No. 2 ·1989-08-00 ·Pages 355-7

Pon CL, Brombach M, Thamm S, Gualerzi CO

Abstract

Using two synthetic deoxyribonucleotide probes encoding segments of the primary structure of initiation factor IF3 from Bacillus stearothermophilus, we identified and cloned a segment of DNA which carries the infC gene. As in Escherichia coli, the infC gene begins with the unusual initiation triplet AUU, and is followed by the structural genes for ribosomal proteins L35 and L20 (rpmI and rplT, respectively).

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Blotting, Southern Cloning, Molecular Genes Genes, Bacterial Geobacillus stearothermophilus/genetics Molecular Sequence Data Multigene Family Oligonucleotide Probes Restriction Mapping Ribosomal Proteins/genetics
Chemicals
Bacterial Proteins Oligonucleotide Probes Ribosomal Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pon C L
Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann, Berlin.
Brombach M
Thamm S
Gualerzi C O
References (10)
10 references, click to expand
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  2. Escherichia coli translational initiation factor IF3: a unique case of translational regulation.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7061-5 PMID: 6390429
  3. Identification, cloning, nucleotide sequence and chromosomal map location of hns, the structural gene for Escherichia coli DNA-binding protein H-NS.
    Mol Gen Genet. 1988 May;212(2):199-202 PMID: 2841565
  4. The primary structure of initiation factor IF3 from Bacillus stearothermophilus.
    FEBS Lett. 1983 Aug 22;160(1-2):78-81 PMID: 6884517
  5. A specialized transducing lambda phage carrying the Escherichia coli genes for phenylalanyl-tRNA synthetase.
    Mol Gen Genet. 1977 Apr 29;152(3):205-10 PMID: 327276
  6. The primary structure of protein L20 from the large subunit of the Escherichia coli ribosome.
    FEBS Lett. 1979 Jul 1;103(1):61-5 PMID: 381019
  7. Transcriptional patterns for the thrS-infC-rplT operon of Escherichia coli.
    Gene. 1988 Mar 31;63(2):309-20 PMID: 2838394
  8. Gene organization around the phenylalanyl-transfer ribonucleic acid synthetase locus in Escherichia coli.
    J Bacteriol. 1981 Apr;146(1):269-74 PMID: 7012115
  9. Primary structures of and genes for new ribosomal proteins A and B in Escherichia coli.
    J Biochem. 1987 Mar;101(3):817-20 PMID: 3298224
  10. Specialized transducing phage for the initiation factor 3 gene in Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3970-4 PMID: 333453
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331290
Published
1989-08-00
Pages
355-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X16188
External Links
PubMed source DOI: 10.1007/BF00331290
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