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PMID: 2783489 Published · ppublish English Case Reports Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular analysis of a t(14;14) translocation in leukemic T-cells of an ataxia telangiectasia patient.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 2 ·1989-01-00 ·Pages 602-6

Russo G, Isobe M, Gatti R, Finan J, Batuman O, Huebner K, Nowell PC, Croce CM

Abstract

We have detected and cloned two rearrangements in the T-cell receptor alpha locus from a clone of somatic cell hybrids carrying a t(14;14)(q11;q32) chromosomal translocation derived from an ataxia telangiectasia patient with T-cell chronic lymphocytic leukemia. The T-cell clone carrying the t(14;14) chromosomal translocation was known to be present for greater than 10 years before the onset of overt leukemia. One molecular rearrangement of the T-cell receptor alpha locus corresponded to a functional variable-joining region (V-J) joining, whereas the other derived from the breakpoint of the t(14;14)(q11;q32) translocation. Chromosomal in situ hybridization of the probe derived from the t(14;14) breakpoint localized the breakpoint region to 14q32.1, apparently the same region that is involved in another ataxia telangiectasia characteristic chromosome translocation, t(7;14)(q35;q32). The 14q32.1 breakpoint is at least 10,000 kilobase pairs (kbp) centromeric to the immunoglobulin heavy chain locus. Sequence analysis of the breakpoint indicates the involvement of a J alpha sequence during the translocation. Comigration of high-molecular weight DNA fragments involved with t(7;14) and t(14;14) translocations suggests the presence of a cluster of breakpoints in the 14q32.1 region, the site of a putative oncogene, TCL1.

MeSH Terms
Alleles Ataxia Telangiectasia/complications,genetics Autoradiography Base Sequence Blotting, Southern Chromosomes, Human, Pair 14 Cloning, Molecular DNA/genetics DNA Probes Female Humans Leukemia, Prolymphocytic, T-Cell/complications,genetics Middle Aged Molecular Sequence Data Nucleic Acid Hybridization Restriction Mapping T-Lymphocytes/ultrastructure Translocation, Genetic
Chemicals
DNA Probes DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Russo G
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140.
Isobe M
Gatti R
Finan J
Batuman O
Huebner K
Nowell P C
Croce C M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.2.602
Published
1989-01-00
Pages
602-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286520
Subset
IM
Grants
NCI NIH HHS · CA 39860 · United States
NCI NIH HHS · CA25875 · United States
Databases
GENBANK
M23431, M23432
External Links
PubMed source DOI: 10.1073/pnas.86.2.602 PMC Full Text View journal details
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