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PMID: 2784570 Published · ppublish English Journal Article

Tumor necrosis factor alpha induces expression of human immunodeficiency virus in a chronically infected T-cell clone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 7 ·1989-04-00 ·Pages 2365-8

Folks TM, Clouse KA, Justement J, Rabson A, Duh E, Kehrl JH, Fauci AS

Abstract

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin, was demonstrated to induce the expression of human immunodeficiency virus (HIV) in a chronically infected T-cell clone (ACH-2). Concentrations of recombinant TNF-alpha as low as 50 pg/ml induced a significant increase over background of HIV expression in the ACH-2 cells as determined by supernatant reverse transcriptase activity. The HIV-inducing effects of TNF-alpha could not be explained by toxic effects on the cells. In addition, both the uninfected parental cell line (A3.01) and the infected ACH-2 cells were shown to have high-affinity receptors for TNF-alpha. Transient-transfection experiments demonstrated that the inductive effects of TNF-alpha were due to specific activation of the HIV long terminal repeat. These studies provide evidence that TNF-alpha may play a role in the mechanisms of pathogenesis of HIV infection.

MeSH Terms
Cell Line Cell Survival/drug effects Clone Cells Gene Expression Regulation/drug effects Genes, Viral/drug effects HIV-1/drug effects,genetics,growth & development Humans Promoter Regions, Genetic/drug effects Recombinant Proteins/pharmacology T-Lymphocytes/drug effects,immunology,microbiology Transfection Tumor Necrosis Factor-alpha/pharmacology
Chemicals
Recombinant Proteins Tumor Necrosis Factor-alpha
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Folks T M
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Clouse K A
Justement J
Rabson A
Duh E
Kehrl J H
Fauci A S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.7.2365
Published
1989-04-00
Pages
2365-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286913
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.7.2365 PMC Full Text View journal details
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