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PMID: 2789518 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Rearrangement by inversion of a T-cell receptor delta variable region gene located 3' of the delta constant region gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 1 ·1989-01-00 ·Pages 267-71

Korman AJ, Maruyama J, Raulet DH

Abstract

We have located a T-cell receptor variable (V) delta gene segment immediately 3' of the delta constant (C) region gene and 5' to the known joining (J) alpha gene segments. This V delta gene is in the opposite transcriptional polarity to C delta and has rearranged to C delta by inversion in a gamma/delta-expressing hybridoma, DN7.3. This V delta gene is commonly rearranged in adult but not fetal gamma/delta-expressing thymocytes and has not been observed among alpha gene rearrangements reported to date. The reciprocal joining sequence isolated from this cell line contains N region nucleotides between the recombination signal sequences, in contrast to previously analyzed reciprocal joints. The results are discussed in the context of models accounting for ordered V gene usage during lymphocyte development.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Southern Cell Line Chromosome Inversion Cloning, Molecular Gene Rearrangement, T-Lymphocyte Genes Genetic Variation Macromolecular Substances Molecular Sequence Data Receptors, Antigen, T-Cell/genetics Restriction Mapping Transcription, Genetic
Chemicals
Macromolecular Substances Receptors, Antigen, T-Cell
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Korman A J
Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142.
Maruyama J
Raulet D H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.1.267
Published
1989-01-00
Pages
267-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286445
Subset
IM
Grants
PHS HHS · A1-26380-01 · United States
NCI NIH HHS · CA28900 · United States
Databases
GENBANK
M23095, M23096, M23097, M23098, M23235
External Links
PubMed source DOI: 10.1073/pnas.86.1.267 PMC Full Text View journal details
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